TY - JOUR
T1 - Anticancer activity study of ethanol extract of Mahkota dewa fruit pulp (Phaleria macrocarpa (Scheff.) Boerl.) in C3H mouse mammary tumor induced by transplantation
AU - Rahmawati, Erni
AU - Dewoto, Hedi R.
AU - Wuyung, Puspita Eka
N1 - Publisher Copyright:
© 2006 Faculty of Medicine, Universitas Indonesia. All rights reserved.
PY - 2006/10/1
Y1 - 2006/10/1
N2 - The objective of this study is to investigate the anticancer activity of 70% ethanol extract of Mahkota dewa fruit pulp [Phaleria macrocarpa (Scheff.) Boerl.] using C3H mouse mammary tumor induced by transplantation. Thirty two C3H mice were devided into 4 groups i.e. control and 3 groups of mice treated with ethanol extract of Mahkota dewa fruit pulp with the dose of 20, 40, and 80 fold human dose respectively, given orally by gastric tube after tumor transplantation for 30 days. Body weight and tumor volume measured twice a week. Tumor weight was measured after the animal was sacrificed, then fixed in formaldehyde for making histopathological preparation. The proliferation activity of tumor cells were examined by counting the AgNOR deposits detected after colloidal AgNOR staining. Apoptosis was assessed by mean of Tunel staining, and the width of necrotic area was identified by hematoxyllin eosin staining of the histological specimen. The results of the study showed that there were no statistical differences in tumor volumes, tumor weights, AGNOR values, and the necrotic area among control and the three treated groups (p>0,05), but apoptosis index significantly increased in the D3 (Mahkota dewa extract of eighty fold human dose) group (p<0,05). It was concluded that ethanol extract of Mahkota dewa fruit pulp at the dose of 20,40, and 80 fold human dose given orally after tumor transplantation for 30 days, did not inhibit the C3H mouse mammary tumor growth induced by transplantation, but the increased apoptosis was found in the group receiving ethanol extract of Mahkota dewa fruit pulp at the dose of 80 fold human dose.
AB - The objective of this study is to investigate the anticancer activity of 70% ethanol extract of Mahkota dewa fruit pulp [Phaleria macrocarpa (Scheff.) Boerl.] using C3H mouse mammary tumor induced by transplantation. Thirty two C3H mice were devided into 4 groups i.e. control and 3 groups of mice treated with ethanol extract of Mahkota dewa fruit pulp with the dose of 20, 40, and 80 fold human dose respectively, given orally by gastric tube after tumor transplantation for 30 days. Body weight and tumor volume measured twice a week. Tumor weight was measured after the animal was sacrificed, then fixed in formaldehyde for making histopathological preparation. The proliferation activity of tumor cells were examined by counting the AgNOR deposits detected after colloidal AgNOR staining. Apoptosis was assessed by mean of Tunel staining, and the width of necrotic area was identified by hematoxyllin eosin staining of the histological specimen. The results of the study showed that there were no statistical differences in tumor volumes, tumor weights, AGNOR values, and the necrotic area among control and the three treated groups (p>0,05), but apoptosis index significantly increased in the D3 (Mahkota dewa extract of eighty fold human dose) group (p<0,05). It was concluded that ethanol extract of Mahkota dewa fruit pulp at the dose of 20,40, and 80 fold human dose given orally after tumor transplantation for 30 days, did not inhibit the C3H mouse mammary tumor growth induced by transplantation, but the increased apoptosis was found in the group receiving ethanol extract of Mahkota dewa fruit pulp at the dose of 80 fold human dose.
KW - Anticancer activity
KW - Apoptosis
KW - Mahkota dewa
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=85008608239&partnerID=8YFLogxK
U2 - 10.13181/mji.v15i4.237
DO - 10.13181/mji.v15i4.237
M3 - Article
AN - SCOPUS:85008608239
SN - 0853-1773
VL - 15
SP - 217
EP - 222
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
IS - 4
ER -