Antibacterial Testing on Silver/Zinc Oxide Nanoparticles/Organoclay Reinforced Chitosan Biocomposites

Lisna Junaeni Muiz, Ariadne Lakshmidevi Juwono, Zulkarnaen Paputungan, Yuni Krisyuningsih Krisnandi

Research output: Contribution to journalArticlepeer-review


Herein, bionanocomposites of chitosan (CS)/silver nanoparticle/organoclay/zinc oxide nanoparticle (CS/Ag/OC/ZnO) were prepared for antibacterial food packaging. This study examines the time variation in the AgNP synthesis method by comparing local (74˗85% deacetylated) and commercial chitosan (75%˗85% deacetylated) as a reducing and capping agent and seeks to reconstruct the optimum ratio formulations of AgNPs and ZnONPs in bionanocomposites for food packaging. The results reveal that the synthesis of AgNPs was successfully carried out using a local chitosan solution as a reducing and capping agent. The CS/Ag/OC/ZnO films exhibit structural, mechanical, and optical properties suitable for food packaging and antibacterial activity on Staphylococcus aureus and Escherichia coli. The relative inhibition zone increased with increasing numbers of AgNPs and ZnONPs. The inhibition zone values indicated that E. coli and S. aureus bacteria were sensitive to the film, namely 12.5 ± 1.5 mm, and 16 ± 0.0 mm, respectively.

Original languageEnglish
Article number9
Pages (from-to)235-246
Number of pages12
JournalMakara Journal of Science
Issue number3
Publication statusPublished - Sept 2023


  • chitosan
  • organoclay
  • silver
  • zinc oxide


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