Phospholipase A2 isolated from venoms is a potential new antibacterial source. Antibacterial activity tests of phospholipase A2 were performed against Staphylococcus aureus and Bacillus subtilis. Phospholipase A2 which was isolated from spines venom of Acanthaster planci by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A2disc against Staphylococcus aureus while no inhibition zone against Bacillus subtilis. As a result, phospholipase A2 posses an antibacterial activity against Staphylococcus aureus.
|Journal||International Journal of Pharma and Bio Sciences|
|Publication status||Published - 1 Jun 2013|
- Acanthaster planci
- Antibacterial activity
- Phospholipase A