Antibacterial investigated of phospholipase A2from the spines venom of crown of thorns starfish Acanthaster planci

R. Fera Ibrahim; Nunuk Widhyastuti; Imelda Krisanta Enda Savitri; Muhamad Sahlan; Anondho Wijanarko

Antibacterial investigated of phospholipase A₂from the spines venom of crown of thorns starfish Acanthaster planci

R. Fera Ibrahim, Nunuk Widhyastuti, Imelda Krisanta Enda Savitri, Muhamad Sahlan, Anondho Wijanarko

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Phospholipase A₂ isolated from venoms is a potential new antibacterial source. Antibacterial activity tests of phospholipase A₂ were performed against Staphylococcus aureus and Bacillus subtilis. Phospholipase A₂ which was isolated from spines venom of Acanthaster planci by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A₂disc against Staphylococcus aureus while no inhibition zone against Bacillus subtilis. As a result, phospholipase A₂ posses an antibacterial activity against Staphylococcus aureus.

Original language	English
Pages (from-to)	B1-B5
Journal	International Journal of Pharma and Bio Sciences
Volume	4
Issue number	2
Publication status	Published - Jun 2013

Keywords

Acanthaster planci
Antibacterial activity
Phospholipase A
Venom

Cite this

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title = "Antibacterial investigated of phospholipase A2from the spines venom of crown of thorns starfish Acanthaster planci",

abstract = "Phospholipase A2 isolated from venoms is a potential new antibacterial source. Antibacterial activity tests of phospholipase A2 were performed against Staphylococcus aureus and Bacillus subtilis. Phospholipase A2 which was isolated from spines venom of Acanthaster planci by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A2disc against Staphylococcus aureus while no inhibition zone against Bacillus subtilis. As a result, phospholipase A2 posses an antibacterial activity against Staphylococcus aureus.",

keywords = "Acanthaster planci, Antibacterial activity, Phospholipase A, Venom",

author = "Ibrahim, {R. Fera} and Nunuk Widhyastuti and Savitri, {Imelda Krisanta Enda} and Muhamad Sahlan and Anondho Wijanarko",

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T1 - Antibacterial investigated of phospholipase A2from the spines venom of crown of thorns starfish Acanthaster planci

AU - Ibrahim, R. Fera

AU - Widhyastuti, Nunuk

AU - Savitri, Imelda Krisanta Enda

AU - Sahlan, Muhamad

AU - Wijanarko, Anondho

PY - 2013/6

Y1 - 2013/6

N2 - Phospholipase A2 isolated from venoms is a potential new antibacterial source. Antibacterial activity tests of phospholipase A2 were performed against Staphylococcus aureus and Bacillus subtilis. Phospholipase A2 which was isolated from spines venom of Acanthaster planci by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A2disc against Staphylococcus aureus while no inhibition zone against Bacillus subtilis. As a result, phospholipase A2 posses an antibacterial activity against Staphylococcus aureus.

AB - Phospholipase A2 isolated from venoms is a potential new antibacterial source. Antibacterial activity tests of phospholipase A2 were performed against Staphylococcus aureus and Bacillus subtilis. Phospholipase A2 which was isolated from spines venom of Acanthaster planci by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A2disc against Staphylococcus aureus while no inhibition zone against Bacillus subtilis. As a result, phospholipase A2 posses an antibacterial activity against Staphylococcus aureus.

KW - Acanthaster planci

KW - Antibacterial activity

KW - Phospholipase A

KW - Venom

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ER -

Antibacterial investigated of phospholipase A₂from the spines venom of crown of thorns starfish Acanthaster planci

Abstract

Keywords

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