TY - JOUR
T1 - Antibacterial effects of sarang semut (Myrmecodia Pendans) fractions using three different solvents toward enterococcus faecalis CPS2
AU - Binartha, Ciptadhi Tri Oka
AU - Suprastiwi, Endang
AU - Kurnia, Didik
AU - Margono, Anggraini
AU - Artiningsih, Dewa Ayu Nyoman Putri
N1 - Publisher Copyright:
© 2020 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
PY - 2020/3
Y1 - 2020/3
N2 - Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane), semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2. Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groups of solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using a phytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five different concentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% was used as a positive control. The width of the inhibition zone was calculated. Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and 19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from the n-hexane and water solvents in inhibiting the growth of E. faecalis cps2. Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of the fraction increased with an increase in concentration.
AB - Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane), semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2. Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groups of solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using a phytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five different concentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% was used as a positive control. The width of the inhibition zone was calculated. Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and 19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from the n-hexane and water solvents in inhibiting the growth of E. faecalis cps2. Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of the fraction increased with an increase in concentration.
KW - Cps2
KW - Enterococcus faecalis
KW - Ethyl acetate
KW - Fraction
KW - Minimum inhibitory concentration
KW - N-hexane
KW - Sarang semut
KW - Water
UR - http://www.scopus.com/inward/record.url?scp=85084142387&partnerID=8YFLogxK
U2 - 10.22159/ijap.2020.v12s1.iwdr01
DO - 10.22159/ijap.2020.v12s1.iwdr01
M3 - Article
AN - SCOPUS:85084142387
SN - 0975-7058
VL - 12
SP - 271
EP - 275
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - Special Issue 1
ER -