Antibacterial effects of sarang semut (Myrmecodia Pendans) fractions using three different solvents toward enterococcus faecalis CPS2

Ciptadhi Tri Oka Binartha, Endang Suprastiwi, Didik Kurnia, Anggraini Margono, Dewa Ayu Nyoman Putri Artiningsih

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Abstract

Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane), semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2. Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groups of solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using a phytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five different concentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% was used as a positive control. The width of the inhibition zone was calculated. Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and 19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from the n-hexane and water solvents in inhibiting the growth of E. faecalis cps2. Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of the fraction increased with an increase in concentration.

Original languageEnglish
Pages (from-to)271-275
Number of pages5
JournalInternational Journal of Applied Pharmaceutics
Volume12
Issue numberSpecial Issue 1
DOIs
Publication statusPublished - Mar 2020

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Keywords

  • Cps2
  • Enterococcus faecalis
  • Ethyl acetate
  • Fraction
  • Minimum inhibitory concentration
  • N-hexane
  • Sarang semut
  • Water

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