Antibacterial effects of 0.1% chlorine dioxide on actinomyces sp. as an agent of black stain

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Objective: This study aimed to assess the antibacterial effects of 0.1% chlorine dioxide and 0.1% chlorhexidine mouthrinses on the bacterial viability of Actinomyces sp. as an agent of black stain. Methods: The authors conducted a clinical trial involving 16 children ages 6–11 with at least 8 black-stained teeth. Subjects were randomized into 2 groups and instructed to rinse with chlorine dioxide or chlorhexidine mouthrinse twice daily. At baseline and after 7 days, samples of black stain plaque were collected, and Actinomyces sp. was cultured. Its bacterial viability was evaluated using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay. Results: After 7 days, Actinomyces sp. viability was remarkably reduced in both groups, and there was a significantly higher reduction in viability in the 0.1% chlorine dioxide group than there was in the 0.1% chlorhexidine group. Conclusion: Mouthrinse containing 0.1% chlorine dioxide has a greater antibacterial effect against Actinomyces sp. than mouthrinse containing 0.1% chlorhexidine.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Issue numberSpecial Issue 2
Publication statusPublished - Dec 2017


  • Actinomyces sp
  • Bacterial viability
  • Black stain
  • Chlorhexidine
  • Chlorine dioxide


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