Antibacterial effectiveness of 2% chitosan and 2% chlorhexidine against enterococcus faecalis in biofilm (Laboratory experiment)

Objective: Enterococcus faecalis can form biofilms and has a major role in the etiology of persistent lesions after root canal. We analyzed the efficacy of chitosan and chlorhexidine against E. faecalis in biofilms. Methods: Polymerase chain reaction was used to analyze E. faecalis DNA that survived and lived after immersing the biofilm in an antibacterial solution. Results: A statistically significant difference was noted in living E. faecalis between chitosan and control and between 2% chlorhexidine and control groups (p≤0.05). No significant difference was noted between chitosan and chlorhexidine groups (p>0.05). Conclusions: Antibacterial effectivity of chitosan is equal to that of chlorhexidine against E. faecalis in biofilm.