Antibacterial effect of virgin coconut oil on (Actinomyces sp.) that causes dental black stain in children

Priscilla Lavine, Eva Fauziah, Mochamad Fahlevi Rizal, Sarworini B. Budiardjo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Objective: This study aimed to assess the effectiveness of virgin coconut oil (VCO) as a natural antibacterial agent to reduce the viability of the chromogenic bacteria Actinomyces sp., which causes dental black stains. Methods: Actinomyces sp. was isolated from the saliva of a child diagnosed with black stain. Each streak of bacteria was cultured on a selective medium Actinomyces agar and confirmed visually and through a gram staining procedure. Each bacterial culture was exposed to VCO in concentrations of 12.5%, 25%, 50%, and 100%. Afterward, viability testing with a methyl-thiazolyl-tetrazolium assay was conducted, and the results were read using an enzyme-linked immunosorbent assay reader. Results: The reduction of bacterial viability of Actinomyces sp. showed a significant difference between the negative control group and the groups treated with various concentrations of VCO 12.5%, 25%, 50%, and 100%. Conclusion: The minimum concentration of VCO necessary to kill Actinomyces sp. is VCO 12.5%.

Original languageEnglish
Pages (from-to)333-335
Number of pages3
JournalAsian Journal of Pharmaceutical and Clinical Research
Issue number2
Publication statusPublished - Feb 2018


  • Actinomyces sp
  • Dental black stain
  • Virgin coconut oil


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