Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima

Rina Hidayati Pratiwi, Iman Hidayat, Muhammad Hanafi, Wibowo Mangunwardoyo

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This study’s aim was to determine the identity of antibacterial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds’ mechanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was observed morphologically through scanning electron microscopy (SEM). This study successfully identified the (2E,5E)-phenyltetradeca-2,5-dienoate antibacterial compound (molecular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca-2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalJournal of Microbiology
Volume55
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

Keywords

  • antibacterial
  • bioactive
  • endophytic bacteria
  • Neesia altissima
  • Pseudomonas aeruginosa strain UICC B-40

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