Antibacterial activity test of the partially purified bromelain from pineapple core extract (Ananas comosus [L.] Merr) by fractionation using ammonium sulfate acetone

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bromelain is a protease, commercially derived from pineapple fruit or stem. In this study, bromelain was isolated from pineapple core (Ananas comosus [L.] Merr) then purified by fractionation using ammonium sulfate and acetone. The fractionated bromelain antibacterial activity was assayed against Staphylococcus epidermidis, Propionibacterium acnes, Staphylococcus aureus and Escherichia coli by disc diffusion method. The highest specific activity and purity was observed at the Fraction 2 ammonium sulfate with saturation level of 20-50%, and Fraction 3 acetone with 50-80% saturation level. The specific activity of Fraction 2 ammonium sulfate is 70 U/mg with a purity level of 5.3 fold compared to its crude extract. Meanwhile, the specific activity of the Fraction 3 acetone is 19.736 U/mg with a purity level of 2.5 fold compared to its crude extract. The antibacterial potency test showed that crude extract of bromelain has an antibacterial activity for all the tested bacteria. The antibacterial potency of the fractionated bromelain is only seen in P. acnes and S. aureus. Based on the antibacterial potency test results, fractionation using acetone can produce bromelain fraction with a better antibacterial activity compared to the fractionation using ammonium sulfate.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • acetone
  • ammonium sulfate
  • antibacterial
  • bromelain
  • fractionation
  • pineapple core

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    Hidayat, Y., Hermawati, E., Setiasih, S., Pws, S. H., & Saefudin, E. (2018). Antibacterial activity test of the partially purified bromelain from pineapple core extract (Ananas comosus [L.] Merr) by fractionation using ammonium sulfate acetone. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 [020067] (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064064