Anti oral biofilm mouthwash nanoemulsion containing extract propolis and curcumin

Muhamad Sahlan, Anissa Permatadietha Ardiellaputri, Heri Hermasnyah, Anondho Wijanarko, Risqa Rina Darwita

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Citations (Scopus)


Background:In the previous research propolis and curcumin have been reported to have biological activity as antibacterial. Both of those herbs can be developed as anti-oral biofilm mouthwash oral hygiene. Mouthwash is produced as nanoemulsion that promotes wide distribution throughout oral biofilm and effectively penetrates to target cell. Objective: This study aims to create the best mouthwash formulation with great physical characteristics and stability, and also proved as an anti-biofilm agent. Methods: Each propolis A.mellifera and curcumin Curcuma domestica Val. was formulated into a mouthwash using the combined method of homogenization, mixing and ultrasonication. There was a variation amount of Tween-80 and glycerin in each formulation to investigate its effect on stability. The proven formula with the greatest stability was continued to undergo antibiofilm assay. Results: Result of this study showed that formula with a ratio of Tween-80 and glycerin 2:1 (v/v) was found to be the best. Tween-80 and glycerin can't work as a single surfactant to produce stable nanoemulsion. Propolis and curcumin mouthwash showed in vitro antibiofilm activities against Streptococcus mutans, the primer colony in the biofilm. Propolis mouthwash reported has better effectiveness with the MIC of biofilm formation was 5% v/v and % inhibition of 48,54%, respectively. Conclusion:Nanoemulsion propolis mouthwash is proven to have the higher effectiveness in inhibiting biofilm formation than curcumin mouthwash. It suggested nanoemulsion propolis can be developed as good oral hygiene mouthwash that cansignificantly reduce the number of oral bacteria and inhibit their proliferation.

Original languageEnglish
Title of host publicationProceeding of the 4th International Symposium on Applied Chemistry, ISAC 2018
EditorsMuhammad Arifuddin Fitriady, Siti Nurul Aisyiyah Jenie, Adid Adep Dwiatmoko
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417441
Publication statusPublished - 7 Nov 2018
Event4th International Symposium on Applied Chemistry, ISAC 2018 - Banten, Indonesia
Duration: 1 Nov 20182 Nov 2018

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Conference4th International Symposium on Applied Chemistry, ISAC 2018


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