Objective: The objectives of this study were to analyze the anti-inflammatory effects of the Lactobacillus casei strain Shirota on epithelial cell expression of interleukin (IL)-8 and human beta-defensin 2 (hBD-2) in the presence of pathogenic bacteria in vitro. Methods: HaCaT epithelial-cells (1 × 105cell/mL) were exposed to heat-killed Streptococcus mutans or Porphyromonas gingivalis (1 × 107 colony-forming units/mL) and then challenged with the probiotic L. casei train Shirota for 3, 6, and 24 h. The mRNA transcription levels of IL-8 and hBD-2 were analyzed by reverse transcription-polymerase chain reaction. In addition, cell viability was analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. Data statistically evaluated by one-way analysis of variance test. Results: L. casei does not effect the HaCaT epithelial cells viability (101.3%). IL-8 and hBD-2 transcription on HaCaT cells was increased after exposed to the heat-killed bacteria. The addition of L. casei reduced the transcription levels of IL-8; in contract, probiotics challenges increased the transcriptional expression of h-BD2 mRNA in the HaCaT cells (P < 0.05). IL-8 expression significantly reduced (P < 0.05) after the L. casei challenge. Conclusion: L. casei conveyed a beneficial immune modulation, reducing IL-8 levels, and elevating production of hBD-2. The epithelial cells showed no cytotoxic effects from L. casei. Further studies are needed to investigate the molecular processes related to this probiotic’s anti-inflammatory properties.
|Number of pages||9415|
|Journal||Drug Invention Today|
|Publication status||Published - 1 Jan 2019|
- Gene expression
- Human beta-defensins-2
- Lactobacillus casei