Anti-elastase activity of methanolic and ethyl acetate extract from Garcinia latissima Miq.

Elastase is a proteinase enzyme that can reduce elastin by dividing specific peptide bonds. Therefore, the inhibition of elastase activity in the dermis layer can be used to maintain skin elasticity. Materials that can inhibit elastase activity can be a cosmetic ingredient in dealing with skin aging. Increased desire to maintain healthy skin without chemicals, encourages the use of materials from natural sources such as plants from Indonesia, a country that has high biodiversity, including Garcinia latissima Miq. from the Clusiaceae family. The purpose of this study was to explore and sought an explanation as to why G. latissima Miq. inhibits elastase enzyme. Plant extraction was carried out using maceration successively methods. The inhibition activity of elastase enzyme was carried out by measuring the kinetic enzyme N-succ-(Ala)-3-Nitroanilide conversion to p-nitroaniline (substrate) spectrophotometry at 405 nm and using porcine pancreatic elastase (PPE) as an enzyme. The results showed that the G. latissima Miq. methanol extract and ethyl acetate extract were active as elastase enzyme inhibitors. G. latissima Miq. extract can maintain skin elasticity.