Antagonistic activity screening of Bacillus siamensisLDR against fungal associated with crop contamination

M. Pertiwi, I. Santoso, Q. G. Fadhilah

Research output: Contribution to journalConference articlepeer-review

Abstract

Bacillus spp. are potential biocontrol agent that commonly used to reduce contamination on crop caused by microorganism, especially fungi. The purpose of this research is to investigate the antagonistic activity of B. siamensis LDR towards fungal crop contaminant (Aspergillus flavus AHM, Aspergillus clavatus ABH, and Aspergillus tamarii KCP). Antagonist assay was conducted using the dual culture method, streak and pour plate technique. The streak technique showed B. siamensis LDR can inhibit the A. flavus AHM, A. clavatus ABH, and A. tamarii KCP with percent inhibition of 42.75 %, 58.89 %, and 28.46 %, respectively. Meanwhile, the pour plate technique represented higher percent inhibition of A. flavus AHM, A. clavatus ABH, and A. tamarii KCP, 89.28 %, 87.32 %, and 66.35 %, respectively.

Original languageEnglish
Article number012002
JournalIOP Conference Series: Earth and Environmental Science
Volume846
Issue number1
DOIs
Publication statusPublished - 4 Oct 2021
Event4th Life and Environmental Sciences Academics Forum, LEAF 2020 - Virtual, Online, Indonesia
Duration: 6 Nov 20207 Nov 2020

Keywords

  • Antagonistic activity
  • Bacillus siamensis LDR
  • dual culture

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