TY - JOUR
T1 - Antagonistic activity screening of Bacillus siamensisLDR against fungal associated with crop contamination
AU - Pertiwi, M.
AU - Santoso, I.
AU - Fadhilah, Q. G.
N1 - Funding Information:
The research was supported by Research Grant awarded from Ministry of Research, Technology and Higher Education basic research funding program to Drs. Iman Santoso, M.Phil. with contract number NKB-2813/UN2.RST/HKP.05.00/2020.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/10/4
Y1 - 2021/10/4
N2 - Bacillus spp. are potential biocontrol agent that commonly used to reduce contamination on crop caused by microorganism, especially fungi. The purpose of this research is to investigate the antagonistic activity of B. siamensis LDR towards fungal crop contaminant (Aspergillus flavus AHM, Aspergillus clavatus ABH, and Aspergillus tamarii KCP). Antagonist assay was conducted using the dual culture method, streak and pour plate technique. The streak technique showed B. siamensis LDR can inhibit the A. flavus AHM, A. clavatus ABH, and A. tamarii KCP with percent inhibition of 42.75 %, 58.89 %, and 28.46 %, respectively. Meanwhile, the pour plate technique represented higher percent inhibition of A. flavus AHM, A. clavatus ABH, and A. tamarii KCP, 89.28 %, 87.32 %, and 66.35 %, respectively.
AB - Bacillus spp. are potential biocontrol agent that commonly used to reduce contamination on crop caused by microorganism, especially fungi. The purpose of this research is to investigate the antagonistic activity of B. siamensis LDR towards fungal crop contaminant (Aspergillus flavus AHM, Aspergillus clavatus ABH, and Aspergillus tamarii KCP). Antagonist assay was conducted using the dual culture method, streak and pour plate technique. The streak technique showed B. siamensis LDR can inhibit the A. flavus AHM, A. clavatus ABH, and A. tamarii KCP with percent inhibition of 42.75 %, 58.89 %, and 28.46 %, respectively. Meanwhile, the pour plate technique represented higher percent inhibition of A. flavus AHM, A. clavatus ABH, and A. tamarii KCP, 89.28 %, 87.32 %, and 66.35 %, respectively.
KW - Antagonistic activity
KW - Bacillus siamensis LDR
KW - dual culture
UR - http://www.scopus.com/inward/record.url?scp=85117935018&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/846/1/012002
DO - 10.1088/1755-1315/846/1/012002
M3 - Conference article
AN - SCOPUS:85117935018
SN - 1755-1307
VL - 846
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012002
T2 - 4th Life and Environmental Sciences Academics Forum, LEAF 2020
Y2 - 6 November 2020 through 7 November 2020
ER -