TY - GEN
T1 - Antagonistic activity of Bacillus siamensis LDR against Curvularia lunata BM
AU - Safitri, Syella Dwi
AU - Santoso, Iman
AU - Fadhilah, Qonita Gina
AU - Maryanto, Andi Eko
AU - Yasman, null
N1 - Funding Information:
The authors are grateful to the Ministry of Research and Technology/National Agency for Research and Innovation for Grant Penelitian Dasar Unggulan Perguruan Tinggi to Iman Santoso, M.Phil (No. NKB-172/UN2.RST/HKP.05.00/2021). We thank you for Collaboration Lab Merck-FMIPA UI for supporting our research.
Publisher Copyright:
© 2023 Author(s).
PY - 2023/6/2
Y1 - 2023/6/2
N2 - Bacillus spp. are known as agricultural biocontrol agents that can produce antifungal compounds. Previous study showed Bacillus siamensis LDR can inhibit several phytopathogenic fungi. In this research, the antagonistic activity of Bacillus siamensis LDR will be evaluated against phytopathogenic fungi, Curvularia lunata BM. The genus Curvularia was reported causing leaf spots and spreading rapidly. This research has isolated black mould from infected coffee leaf (Coffea sp.). The isolation was performed on Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) medium supplemented with chloramphenicol. The result of microscopic and macroscopic observation showed that the characteristics of fungus was referred to genus Curvularia. The molecular identification based on ITS region showed the fungus isolate was belonged to Curvularia lunata with 100% sequence similarity. The antagonist test was carried out using a dual culture method with streak technique. The antagonistic activity of B. siamensis LDR was performed on PDA and compared to modified Czapek-Dox Agar (CDA) using hanjeli (Coix lacryma-jobi) starch as a carbon source. The result showed that B. siamensis LDR was able to inhibit the growth of C. lunata BM on PDA and modified CDA medium. Nevertheless, the percentage inhibition on PDA (63.25%) was higher than on modified CDA medium (42.15%).
AB - Bacillus spp. are known as agricultural biocontrol agents that can produce antifungal compounds. Previous study showed Bacillus siamensis LDR can inhibit several phytopathogenic fungi. In this research, the antagonistic activity of Bacillus siamensis LDR will be evaluated against phytopathogenic fungi, Curvularia lunata BM. The genus Curvularia was reported causing leaf spots and spreading rapidly. This research has isolated black mould from infected coffee leaf (Coffea sp.). The isolation was performed on Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) medium supplemented with chloramphenicol. The result of microscopic and macroscopic observation showed that the characteristics of fungus was referred to genus Curvularia. The molecular identification based on ITS region showed the fungus isolate was belonged to Curvularia lunata with 100% sequence similarity. The antagonist test was carried out using a dual culture method with streak technique. The antagonistic activity of B. siamensis LDR was performed on PDA and compared to modified Czapek-Dox Agar (CDA) using hanjeli (Coix lacryma-jobi) starch as a carbon source. The result showed that B. siamensis LDR was able to inhibit the growth of C. lunata BM on PDA and modified CDA medium. Nevertheless, the percentage inhibition on PDA (63.25%) was higher than on modified CDA medium (42.15%).
UR - http://www.scopus.com/inward/record.url?scp=85163295587&partnerID=8YFLogxK
U2 - 10.1063/5.0140448
DO - 10.1063/5.0140448
M3 - Conference contribution
AN - SCOPUS:85163295587
T3 - AIP Conference Proceedings
BT - 11th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application, ISNPINSA 2021
A2 - Soesanto, Qidir Maulana Binu
A2 - Bima, Damar Nurwahyu
A2 - Prasetya, Nor Basid Adiwibawa
A2 - Sugito, Heri
A2 - Maulidiyah, Alik
PB - American Institute of Physics Inc.
T2 - 11th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application: Developing Science and Technology Toward the New Normal Era, ISNPINSA 2021
Y2 - 14 October 2021 through 15 October 2021
ER -