TY - JOUR
T1 - Antagonist effect of Bacillus spp. Against Aspergillus niger CP isolated from cocopeat powder
AU - Santoso, I.
AU - Fadhilah, Q. G.
AU - Maryanto, A. E.
AU - Yasman, null
N1 - Funding Information:
The research was supported by Ministry of Research, Technology and Higher for Basic Research Grant program (PDUPT) awarded to Drs. Iman Santoso, (No. NKB-2813/UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/10/4
Y1 - 2021/10/4
N2 - Bacillus spp. are known as potential bacteria as biocontrol agent against fungal phytopathogen, such as Aspergillus niger. The Aspergillus niger can cause many problems, including in agriculture sector. Antagonist activity of 3 bacteria isolates Bacillus sp. KRG, KRT and LDR have been carried out against Aspergillus niger CP. Dual culture method for antagonist assay was performed using streak, disc, and pour plate technique. Result from the disc technique showed that there is no significant antagonist activity differences between all isolates KRG, KRT, and LDR (51.94 %, 61.24 %, and 54.53 %). The highest inhibition effect was shown clearly in pour plate technique with inhibition value almost 100 %. The LDR isolate was selected for further evaluation based on the physicochemical characters according to strong catalase activity and rapid fermentation. Antibiosis assay was performed in order to evaluate the antifungal compound produced by LDR isolate using agar and broth culture method. The filtrates from growth medium 7, 10 and 12 days fermentation were used for antibiosis assay against Asp. niger CP. The growth of Asp. niger CP was inhibited by 10- and 12-days filtrate fermentation in all the 2 methods used. The percentage of inhibition of isolates observed in agar culture were 84.41 % and 84.21 %. Biomass of Asp. niger CP reduced by 74.55 % and 85.54 %.
AB - Bacillus spp. are known as potential bacteria as biocontrol agent against fungal phytopathogen, such as Aspergillus niger. The Aspergillus niger can cause many problems, including in agriculture sector. Antagonist activity of 3 bacteria isolates Bacillus sp. KRG, KRT and LDR have been carried out against Aspergillus niger CP. Dual culture method for antagonist assay was performed using streak, disc, and pour plate technique. Result from the disc technique showed that there is no significant antagonist activity differences between all isolates KRG, KRT, and LDR (51.94 %, 61.24 %, and 54.53 %). The highest inhibition effect was shown clearly in pour plate technique with inhibition value almost 100 %. The LDR isolate was selected for further evaluation based on the physicochemical characters according to strong catalase activity and rapid fermentation. Antibiosis assay was performed in order to evaluate the antifungal compound produced by LDR isolate using agar and broth culture method. The filtrates from growth medium 7, 10 and 12 days fermentation were used for antibiosis assay against Asp. niger CP. The growth of Asp. niger CP was inhibited by 10- and 12-days filtrate fermentation in all the 2 methods used. The percentage of inhibition of isolates observed in agar culture were 84.41 % and 84.21 %. Biomass of Asp. niger CP reduced by 74.55 % and 85.54 %.
KW - Antagonistic activity
KW - Aspergillus niger
KW - Bacillus spp
KW - dual culture
KW - identification
UR - http://www.scopus.com/inward/record.url?scp=85117940951&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/846/1/012001
DO - 10.1088/1755-1315/846/1/012001
M3 - Conference article
AN - SCOPUS:85117940951
SN - 1755-1307
VL - 846
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012001
T2 - 4th Life and Environmental Sciences Academics Forum, LEAF 2020
Y2 - 6 November 2020 through 7 November 2020
ER -