Annona muricata leaves ethanolic extract in water, n-hexane, and methanol fractions: Cytotoxicity assay, antioxidant activity, flavonoid content, polyphenol content, and acetogenin content

Elsa Anisa Krisanti, Afidya Indina Harumanti, Kamarza Mulia

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Cytotoxicity, antioxidant activity, and a total bioactive assay of the ethanolic extract of Annona muricata leave partitioned into water, n-hexane, and methanol fractions were investigated. The brine shrimp lethality test used for the cytotoxicity assay gave the LC50 values of 84 and 1 ppm for the n-hexane and methanol fractions, respectively. The antioxidant activity determined using DPPH reagent gave IC50 values of 40, 15, and 8 ppm for the n-hexane, water, and methanol fractions, respectively. In addition, the total polyphenol and the total acetogenin content of the methanol fraction were also the highest among the three fractions. These results indicate that the methanol fraction of the Annona muricata has the highest potential as a source for naturally occurring antitumor and antioxidant compounds.

Original languageEnglish
Title of host publication5th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the 5th International Symposium of Biomedical Engineering, ISBE 2020
EditorsKenny Lischer, Sugeng Supriadi, Siti Fauziyah Rahman, Yudan Whulanza
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735440869
DOIs
Publication statusPublished - 23 Mar 2021
Event5th International Symposium of Biomedical Engineering, ISBE 2020 - Depok, Virtual, Indonesia
Duration: 28 Jul 202029 Jul 2020

Publication series

NameAIP Conference Proceedings
Volume2344
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium of Biomedical Engineering, ISBE 2020
Country/TerritoryIndonesia
CityDepok, Virtual
Period28/07/2029/07/20

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