Angiotensin converting enzyme inhibitory activity of melinjo (Gnetum gnemon L.) seed extracts and molecular docking of its stilbene constituents

Abdul Mun'im, Muhammad Ashar Munadhil, Nuraini Puspitasari, Azminah, Arry Yanuar

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives: To evaluate the angiotensin converting enzyme (ACE) inhibitory activity of melinjo (Gnetum gnemon) seed extract and to study molecular docking of stilbene contained in melinjo seeds. Methods: Melinjo seed powders were extracted with n-hexane, dichloromethane, ethyl acetate, methanol, and water successively. The extracts were evaluated ACE inhibitory activities using ACE kit-Wist and the phenolic content using Folin–Ciocalteu method. The extract demonstrated the highest ACE inhibitory activity was subjected to liquid chromatography-mass spectrometry (LC-MS) to know its stilbene constituent. The stilbene constituents in melinjo seed were performed molecular docking using AutoDock Vina, and ligand-receptor Interactions were processed using Ligand Scout. Results: The ethyl acetate extract demonstrated the highest ACE inhibition activity with inhibitory concentration 50% value of 9.77 × 10−8 μg/mL and the highest total phenolic content (575.9 mg gallic acid equivalent/g). Ultra-performance LC-MS analysis of ethyl acetate extract has detected the existency of resveratrol, gnetin C, ε-viniferin, and gnemonoside A/B. These compounds displayed similar physiochemical properties to lisinopril (ACE inhibitor), as in silico molecular docking studies demonstrated that they fit into the lisinopril receptors. Conclusion: In vitro analysis ethyl acetate extract from melinjo seeds demonstrated the highest ACE inhibitory activity. Molecular docking analysis indicated that resveratrol dimers, gnetin C and gnemonoside A can be considered ACE inhibitor.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume10
Issue number3
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • Angiotensin converting enzyme inhibitor
  • Antihypertension
  • Gnetum gnemon
  • Melinjo
  • Molecular docking
  • Total phenolic

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