Analytical method optimization and validation of glibenclamide and metformin hydrochloride in diabetic herbs product by thin-layer chromatography-densitometry

Objective: This study aimed to develop and validate an analytical method using thin-layer chromatography (TLC)-densitometry for detecting the presence of glibenclamide and metformin hydrochloride in herbal diabetic products. Methods: Thejamu samples were extracted using methanol, followed by analysis using TLC-densitometry with methanol-aquades-glacial acetic acid (9:1:0.25) as the mobile phase at a wavelength of 237 nm. Results: From the validation results, the correlation coefficients for glibenclamide and metformin hydrochloride were determined to be 0.9998 and 0.9981, respectively. The limits of detection and quantitation for glibenclamide were 49.97 ng/mL and 66.55 ng/mL, respectively, while those of metformin hydrochloride were 74.75 ng/mL and 249.25 ng/mL, respectively. The method developed in this study met the criteria of selectivity, accuracy, and precision. From seven jamu samples tested, four tested positive for glibenclamide, with sample 1=4.9522%, sample 2=4.1495%, sample 3=4.2578%, and sample 4=4.9412%. Conclusion: None of the jamu samples contained metformin hydrochloride.