Abstract
Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples. Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol- ethanol-water (4:5:5) and isobutanol-ethanol-water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol-ethanol-water (3:7:1). Results: No synthetic red dyes were found in the seven red spinach samples. Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.
| Original language | English |
|---|---|
| Pages (from-to) | 15-18 |
| Number of pages | 4 |
| Journal | International Journal of Applied Pharmaceutics |
| Volume | 10 |
| Issue number | Special Issue 1 |
| DOIs | |
| Publication status | Published - Dec 2018 |
Keywords
- Amaranth
- Karmoisin
- Paper chromatography
- Ponceau 4R
- Red spinach
- Rhodamin B
- Thin-layer chromatography-densitometry
- Thinlayer chromatography
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