Analysis of synthetic red dyes in red spinach samples (Amaranthus Tricolor L.)

Dolvin Novitasari; Rani Sauriasari; Maryati Kurniadi

doi:10.22159/ijap.2018.v10s1.04

Analysis of synthetic red dyes in red spinach samples (Amaranthus Tricolor L.)

Dolvin Novitasari, Rani Sauriasari, Maryati Kurniadi

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples. Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol- ethanol-water (4:5:5) and isobutanol-ethanol-water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol-ethanol-water (3:7:1). Results: No synthetic red dyes were found in the seven red spinach samples. Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.

Original language	English
Pages (from-to)	15-18
Number of pages	4
Journal	International Journal of Applied Pharmaceutics
Volume	10
Issue number	Special Issue 1
DOIs	https://doi.org/10.22159/ijap.2018.v10s1.04
Publication status	Published - 1 Dec 2018

Keywords

Amaranth
Karmoisin
Paper chromatography
Ponceau 4R
Red spinach
Rhodamin B
Thin-layer chromatography-densitometry
Thinlayer chromatography

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10.22159/ijap.2018.v10s1.04

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title = "Analysis of synthetic red dyes in red spinach samples (Amaranthus Tricolor L.)",

abstract = "Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples. Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol- ethanol-water (4:5:5) and isobutanol-ethanol-water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol-ethanol-water (3:7:1). Results: No synthetic red dyes were found in the seven red spinach samples. Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.",

keywords = "Amaranth, Karmoisin, Paper chromatography, Ponceau 4R, Red spinach, Rhodamin B, Thin-layer chromatography-densitometry, Thinlayer chromatography",

author = "Dolvin Novitasari and Rani Sauriasari and Maryati Kurniadi",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors. Published by Innovare Academic Sciences Pvt Ltd.",

year = "2018",

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doi = "10.22159/ijap.2018.v10s1.04",

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AU - Novitasari, Dolvin

AU - Sauriasari, Rani

AU - Kurniadi, Maryati

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples. Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol- ethanol-water (4:5:5) and isobutanol-ethanol-water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol-ethanol-water (3:7:1). Results: No synthetic red dyes were found in the seven red spinach samples. Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.

AB - Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples. Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol- ethanol-water (4:5:5) and isobutanol-ethanol-water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol-ethanol-water (3:7:1). Results: No synthetic red dyes were found in the seven red spinach samples. Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.

KW - Amaranth

KW - Karmoisin

KW - Paper chromatography

KW - Ponceau 4R

KW - Red spinach

KW - Rhodamin B

KW - Thin-layer chromatography-densitometry

KW - Thinlayer chromatography

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SN - 0975-7058

VL - 10

SP - 15

EP - 18

JO - International Journal of Applied Pharmaceutics

JF - International Journal of Applied Pharmaceutics

IS - Special Issue 1

ER -

Analysis of synthetic red dyes in red spinach samples (Amaranthus Tricolor L.)

Abstract

Keywords

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