Analysis of strain type and quantitative of Enterococcus faecalis bacteria in true combined endo-perio lesions

Enterococcus faecalis bacteria are root canal pathogens that can migrate to periodontal tissue in true combined endo-perio lesions through apical foramen, dentinal tubules, and lateral and accessory canals. The same strain of E. faecalis in root canals and pocket periodonsium tissue in true combined endo-perio lesions showed pathological unity and the possibility of migration. The quantity of E. faecalis contributes to the disease's severity because of its virulence, i.e., substance aggregation, lipoteichoic acid, extracellular superoxide, gelatinase, hyaluronidase, and hemolysin. To analyze the strain type and quantity of the E. faecalis taken from root canals and pockets of true combined endo-perio lesions, primary endodontic lesions, and primary periodontal lesions. 16 samples were taken from the following three diagnosis groups: true combined endo-perio lesions (root canal and pocket), primary endodontic lesions, and primary periodontal lesions. The strain types were analyzed via sequencing methods and quantification real-time PCR. There were similarities of the E. faecalis strains in all groups. The largest number was found in the pockets of true combined endo-perio lesions. There were significant differences between the strains in the root canals in true combined endo-perio lesions and those in the primary endodontic lesions. However, there were no significant differences between the strains in the root canals and pockets in true combined endo-perio lesions and between the strains in pockets of true combined endo-perio lesions and in primary periodontal lesions. There were similarities between the strains, and the quantity of E. faecalis were also equal between the root canals and the pockets in true combined endo-perio lesions.