TY - JOUR
T1 - Analysis of strain type and quantitative of Enterococcus faecalis bacteria in true combined endo-perio lesions
AU - Elvira, Nova
AU - Kamizar, null
AU - Meidyawati, Ratna
N1 - Publisher Copyright:
© 2018, University of Dicle.
PY - 2018
Y1 - 2018
N2 - Enterococcus faecalis bacteria are root canal pathogens that can migrate to periodontal tissue in true combined endo-perio lesions through apical foramen, dentinal tubules, and lateral and accessory canals. The same strain of E. faecalis in root canals and pocket periodonsium tissue in true combined endo-perio lesions showed pathological unity and the possibility of migration. The quantity of E. faecalis contributes to the disease's severity because of its virulence, i.e., substance aggregation, lipoteichoic acid, extracellular superoxide, gelatinase, hyaluronidase, and hemolysin. To analyze the strain type and quantity of the E. faecalis taken from root canals and pockets of true combined endo-perio lesions, primary endodontic lesions, and primary periodontal lesions. 16 samples were taken from the following three diagnosis groups: true combined endo-perio lesions (root canal and pocket), primary endodontic lesions, and primary periodontal lesions. The strain types were analyzed via sequencing methods and quantification real-time PCR. There were similarities of the E. faecalis strains in all groups. The largest number was found in the pockets of true combined endo-perio lesions. There were significant differences between the strains in the root canals in true combined endo-perio lesions and those in the primary endodontic lesions. However, there were no significant differences between the strains in the root canals and pockets in true combined endo-perio lesions and between the strains in pockets of true combined endo-perio lesions and in primary periodontal lesions. There were similarities between the strains, and the quantity of E. faecalis were also equal between the root canals and the pockets in true combined endo-perio lesions.
AB - Enterococcus faecalis bacteria are root canal pathogens that can migrate to periodontal tissue in true combined endo-perio lesions through apical foramen, dentinal tubules, and lateral and accessory canals. The same strain of E. faecalis in root canals and pocket periodonsium tissue in true combined endo-perio lesions showed pathological unity and the possibility of migration. The quantity of E. faecalis contributes to the disease's severity because of its virulence, i.e., substance aggregation, lipoteichoic acid, extracellular superoxide, gelatinase, hyaluronidase, and hemolysin. To analyze the strain type and quantity of the E. faecalis taken from root canals and pockets of true combined endo-perio lesions, primary endodontic lesions, and primary periodontal lesions. 16 samples were taken from the following three diagnosis groups: true combined endo-perio lesions (root canal and pocket), primary endodontic lesions, and primary periodontal lesions. The strain types were analyzed via sequencing methods and quantification real-time PCR. There were similarities of the E. faecalis strains in all groups. The largest number was found in the pockets of true combined endo-perio lesions. There were significant differences between the strains in the root canals in true combined endo-perio lesions and those in the primary endodontic lesions. However, there were no significant differences between the strains in the root canals and pockets in true combined endo-perio lesions and between the strains in pockets of true combined endo-perio lesions and in primary periodontal lesions. There were similarities between the strains, and the quantity of E. faecalis were also equal between the root canals and the pockets in true combined endo-perio lesions.
KW - E. faecalis
KW - Strain and quantity
KW - True combined endo-perio lesion
UR - http://www.scopus.com/inward/record.url?scp=85046481560&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85046481560
SN - 1309-100X
VL - 11
SP - 175
EP - 180
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 1
ER -