Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)

E. L.U. Basa; H. Julendra; A. Abinawanto; A. Sofyan; A. Sophian

doi:10.1063/1.5132522

Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)

E. L.U. Basa, H. Julendra, A. Abinawanto, A. Sofyan, A. Sophian

Department of Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.

Original language	English
Title of host publication	Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
Editors	Terry Mart, Djoko Triyono, Ivandini T. Anggraningrum
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419155
DOIs	https://doi.org/10.1063/1.5132522
Publication status	Published - 4 Nov 2019
Event	4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia Duration: 30 Oct 2018 → 31 Oct 2018

Publication series

Name	AIP Conference Proceedings
Volume	2168
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
Country/Territory	Indonesia
City	Depok
Period	30/10/18 → 31/10/18

Keywords

GC-MS
L. Plantarum
oleic acid

Access to Document

10.1063/1.5132522

Cite this

Basa, E. L. U., Julendra, H., Abinawanto, A., Sofyan, A., & Sophian, A. (2019). Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS). In T. Mart, D. Triyono, & I. T. Anggraningrum (Eds.), Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018 Article 020095 (AIP Conference Proceedings; Vol. 2168). American Institute of Physics Inc.. https://doi.org/10.1063/1.5132522

Basa, E. L.U. ; Julendra, H. ; Abinawanto, A. et al. / Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS). Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018. editor / Terry Mart ; Djoko Triyono ; Ivandini T. Anggraningrum. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

@inproceedings{6a3a2f8f0e3a418bb2c4c369afc8d817,

title = "Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)",

abstract = "A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.",

keywords = "GC-MS, L. Plantarum, oleic acid",

author = "Basa, {E. L.U.} and H. Julendra and A. Abinawanto and A. Sofyan and A. Sophian",

note = "Funding Information: This study was supported by Direktorat Riset dan Pengabdian Masyarakat Universitas Indonesia (DRPM UI) through “Publikasi Internasional Terindeks untuk Tugas Akhir Mahasiswa (PITTA)” Grant. BPTBA-LIPI, Gunung Kidul for the L. plantarum AKK 30 isolates. There was no conflict interest while conducting the research. Publisher Copyright: {\textcopyright} 2019 Author(s). Copyright: Copyright 2019 Elsevier B.V., All rights reserved.; 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 ; Conference date: 30-10-2018 Through 31-10-2018",

year = "2019",

month = nov,

day = "4",

doi = "10.1063/1.5132522",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Terry Mart and Djoko Triyono and Anggraningrum, {Ivandini T.}",

booktitle = "Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018",

address = "United States",

}

Basa, ELU, Julendra, H, Abinawanto, A, Sofyan, A & Sophian, A 2019, Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS). in T Mart, D Triyono & IT Anggraningrum (eds), Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018., 020095, AIP Conference Proceedings, vol. 2168, American Institute of Physics Inc., 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018, Depok, Indonesia, 30/10/18. https://doi.org/10.1063/1.5132522

Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS). / Basa, E. L.U.; Julendra, H.; Abinawanto, A. et al.
Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018. ed. / Terry Mart; Djoko Triyono; Ivandini T. Anggraningrum. American Institute of Physics Inc., 2019. 020095 (AIP Conference Proceedings; Vol. 2168).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)

AU - Basa, E. L.U.

AU - Julendra, H.

AU - Abinawanto, A.

AU - Sofyan, A.

AU - Sophian, A.

N1 - Funding Information: This study was supported by Direktorat Riset dan Pengabdian Masyarakat Universitas Indonesia (DRPM UI) through “Publikasi Internasional Terindeks untuk Tugas Akhir Mahasiswa (PITTA)” Grant. BPTBA-LIPI, Gunung Kidul for the L. plantarum AKK 30 isolates. There was no conflict interest while conducting the research. Publisher Copyright: © 2019 Author(s). Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/11/4

Y1 - 2019/11/4

N2 - A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.

AB - A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.

KW - GC-MS

KW - L. Plantarum

KW - oleic acid

UR - http://www.scopus.com/inward/record.url?scp=85074989067&partnerID=8YFLogxK

U2 - 10.1063/1.5132522

DO - 10.1063/1.5132522

M3 - Conference contribution

AN - SCOPUS:85074989067

T3 - AIP Conference Proceedings

BT - Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018

A2 - Mart, Terry

A2 - Triyono, Djoko

A2 - Anggraningrum, Ivandini T.

PB - American Institute of Physics Inc.

T2 - 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018

Y2 - 30 October 2018 through 31 October 2018

ER -

Basa ELU, Julendra H, Abinawanto A, Sofyan A, Sophian A. Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS). In Mart T, Triyono D, Anggraningrum IT, editors, Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018. American Institute of Physics Inc. 2019. 020095. (AIP Conference Proceedings). doi: 10.1063/1.5132522

Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)

Abstract

Publication series

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this