Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)

E. L.U. Basa, H. Julendra, A. Abinawanto, A. Sofyan, A. Sophian

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
Country/TerritoryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • GC-MS
  • L. Plantarum
  • oleic acid

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