A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.