TY - GEN
T1 - Analysis of organic acids from Lactobacillus plantarum with gas chromatography-mass spectrometry (GC-MS)
AU - Basa, E. L.U.
AU - Julendra, H.
AU - Abinawanto, A.
AU - Sofyan, A.
AU - Sophian, A.
N1 - Funding Information:
This study was supported by Direktorat Riset dan Pengabdian Masyarakat Universitas Indonesia (DRPM UI) through “Publikasi Internasional Terindeks untuk Tugas Akhir Mahasiswa (PITTA)” Grant. BPTBA-LIPI, Gunung Kidul for the L. plantarum AKK 30 isolates. There was no conflict interest while conducting the research.
Publisher Copyright:
© 2019 Author(s).
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/11/4
Y1 - 2019/11/4
N2 - A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.
AB - A descriptive investigation to evaluate the combination of Lactobacillus plantarum and oligosaccharides was studied using gas chromatography-mass spectrometry (GC-MS). Oligosaccharides are major carbohydrate sources for lactobacilli. The samples in nine tubes were divided into three groups based on the added oligosaccharides, control group L. plantarum without oligosaccharides (0 %), four tubes with inulin (I) and four tubes with mannan-oligosaccharides (MOS) in 0.5 %, 1.0 %, 1.5 %, 2.0 % concentration, respectively. The results of GC-MS showed the highest peak of oleic acid. The control group showed oleic acid levels of 51.32 %. Oleic acid decreased in the isolates added inulin (36.47 %). Oleic acid did not increase in isolates with MOS compared to control group, i.e., between 41.70 % and 47.23 %. In conclusion the highest organic acid level from our strain, L. plantarum AKK 30, was oleic acid. However, oligosaccharides which added to isolates had no effect on the oleic acid level.
KW - GC-MS
KW - L. Plantarum
KW - oleic acid
UR - http://www.scopus.com/inward/record.url?scp=85074989067&partnerID=8YFLogxK
U2 - 10.1063/1.5132522
DO - 10.1063/1.5132522
M3 - Conference contribution
AN - SCOPUS:85074989067
T3 - AIP Conference Proceedings
BT - Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
A2 - Mart, Terry
A2 - Triyono, Djoko
A2 - Anggraningrum, Ivandini T.
PB - American Institute of Physics Inc.
T2 - 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
Y2 - 30 October 2018 through 31 October 2018
ER -