Analysis of morphology and absorption of calcium and magnesium for calcium phosphate Ca3(PO4)2 in rat's spine

M. N. Ramahwati, A. L. Juwono, D. S. Soejoko, N. N. Mulyaningsih

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)


The decline in bone mass is a sign of metabolic diseases called osteoporosis. Osteoporosis can be treated by adding the calcium level in the form of nano sized Ca3(PO4)2. One type of calcium which can build and strengthen the bones is Ca3(PO4)2. This study aimed to determine the effectively of nano sized Ca3(PO4)2 addition which can be absorbed by rat bones and to identify the crystalline structure that was formed in rat bones with a specific purified diet. Nano sized Ca3(PO4)2 was varied in a composition 0.5X, 1.0X and 1.5X compared to the normal needs in the purified diet. The rats were harvested in every two weeks, then their spines were taken to be investigated. The rat's spines were examined by using X-Ray Diffraction (XRD) and Atomic Absorption Spectroscopy (AAS). The effective amount of calcium for the rats was at the value of 0.5X. The amount of Ca as a bone mineral increased as the increasing amount of nano sized Ca3(PO4)2, while the amount of Mg tended to fluctuate. The crystalline structure of hexagonal type was found in the rat bones with. Lattice parameters of a = 9.50 A and c = 6.83 A.

Original languageEnglish
Article number012039
JournalIOP Conference Series: Materials Science and Engineering
Issue number1
Publication statusPublished - 22 Feb 2019
Event2nd International Conference on Current Progress in Functional Materials 2017, ISCPFM 2017 - Bali, Indonesia
Duration: 8 Nov 20179 Nov 2017


  • atomic absorption spectroscopy
  • Ca(PO)
  • hydroxyapatite
  • purified diet
  • X-Ray Diffraction


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