Analysis of lysate platelet-rich fibrin effects on human dental pulp stem cell differentiation through dentin sialophosphoprotein expression

Silviana Swastiningtyas, Anggraini Margono, Dini Asrianti, Runy Oktayani, Indah Yulianto

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: In vitro, the culture media in which human dental pulp stem cells (hDPSCs) are grown are supplemented with specific growth factors that induce cell cycle entry and differentiation. Lysate platelet-rich fibrin (L-PRF) is a unique and stable growth factor supplement produced from platelets lysed by freezing-thawing. In this study, we aimed to analyze the potential effects of L-PRF on hDPSC differentiation. Methods: We divided hDPSCs isolated from human third molars at the second passage into five culture media groups treated with 1%, 5%, 10%, and 25% L-PRF or 10% fetal bovine serum (control). After 7 days, we evaluated hDPSC differentiation using an enzyme-linked immunosorbent assay specific for dentin sialophosphoprotein and Alizarin-Red staining. Results: None of our analyses revealed any significant differences between the L-PRF-and control-treated cells. Conclusion: L-PRF could potentially induce the differentiation of hDPSCs in vitro.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Volume12
Issue numberSpecial Issue 2
DOIs
Publication statusPublished - Jul 2020

Keywords

  • Dentin sialophosphoprotein
  • Differentiation
  • Human dental pulp stem cells
  • Lysate platelet-rich fibrin

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