Knowledge is one of the most valuable assets for an organization. By managing existing knowledge, organizations can gain competitive advantage in competition with other organizations. Otherwise, if the existing knowledge within the organization is not properly managed, it can lead to losses such as loss of business opportunities, and the destruction of the organization due to lack of innovation so that knowledge management is required. In practice, knowledge management is not always successfully implemented by every organization. To be able to reduce the risk of failure in the effort of knowledge management, assessment of knowledge management readiness implementation is required in the organization. The purpose of this research is to know the readiness of knowledge management implementation and its factors that can be improved in the IT Service company. Aspects used in this research are Organization Culture, Organization Structure, IT Infrastructure, Individual Acceptance and Intention to be involved in KM Process. The methodology used in this study used a survey approach on 103 respondents with reliability and validity test in data processing and assessment of readiness and PLS for factor analysis. This research concludes that the company has reached level 4 or receptive readiness level. With a factor that significantly affects employees to be involved in the KM process are learning, business strategy, reward, IT uses, and effort expectancy.