Analysis of Droplet Size Control for Stem Cells Encapsulation using Lab-on-a-chip

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

In in-vivo bone tissue engineering, therapeutic efficacies are often unsatisfactory because the stem cells lose significant viability post-transplantation. The damaged cell may also present an unfavorable environment for cell growth. This means that cell transplantation would require the cells to be kept in a particular environment with substances that can support their survival, induce bioactivity, and enhance retention at the administered sites. This need drives the development of stem cells encapsulation in a biomaterial-based microcapsule. Our previous study has been conducted to encapsulate mesenchymal stem cells (MSC) in a feasible laboratory setting. It showed the survival of the MSC in the alginate system. However, higher throughput and uniform size of the encapsulated cells are needed. Therefore, this study introduces a Lab-on-a-chip setup to produce encapsulated stem cells as required quality. Here, an initial study on droplet generation was carried out using Computational Fluid Dynamics software. The study will use the volume of fluid methods with multiphase calculation taking oil as primary phase and water as a secondary phase. The CFD simulation shows that droplet size can be controlled at the range of 100-400 μm by varying the flow rate parameters. A comparative study also showed to confirm our result. This paper can be used for further studies in controllable droplet generation for stem cell encapsulation.

Original languageEnglish
Title of host publication6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021
EditorsSiti Fauziyah Rahman, Ahmad Zakiyuddin, Yudan Whulanza, Nurul Intan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735443716
DOIs
Publication statusPublished - 16 Aug 2022
Event6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021 - Depok, Virtual, Indonesia
Duration: 7 Jul 20218 Jul 2021

Publication series

NameAIP Conference Proceedings
Volume2537
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Country/TerritoryIndonesia
CityDepok, Virtual
Period7/07/218/07/21

Keywords

  • droplet
  • microfluidics
  • stem cell

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