TY - JOUR
T1 - Analysis of CD4 and CD8 expression in multidrug-resistant tuberculosis infection with diabetes mellitus
T2 - An experimental study in mice
AU - Agustin, Heidy
AU - Massi, Muhammad Nasrum
AU - Djaharuddin, Irawati
AU - Susanto, Agus Dwi
AU - Islam, Andi Asadul
AU - Hatta, Mochammad
AU - Bukhari, Agussalim
AU - Tabri, Nur Ahmad
AU - Santoso, Arif
AU - Patellongi, Ilhamjaya
N1 - Funding Information:
We acknowledge Muhammad Faruk, M.D, for his help in providing us with the linguistic assistance for this experimental study.
Publisher Copyright:
© 2021 The Authors
PY - 2021/8
Y1 - 2021/8
N2 - Background: Tuberculosis (TB) remains a major global health problem, in the top 10 causes of death. As a regulator of the immune response, T-helper (Th) cells activate other lymphocytes from the immune system, such as B cells, to destroy the TB pathogen by releasing CD4 and CD8 Th cells. Diabetes mellitus (DM) is a known cause of developing active pulmonary TB. Few studies have examined the biomolecular expression affecting Mycobacterium tuberculosis (MTB) and multidrug-resistant (MDR) MTB, which are associated with low immunity represented by TB in diabetes and CD4 and CD8 levels. Materials and methods: This animal study used a post-test control group design. We performed an experimental study using 30 BALB/c mice, each weighing 25 g. It included six experimental animal groups, of which three had a diabetes condition induced using intraperitoneal streptozotocin, and all were infected with MTB or MDR TB. We evaluated the CD4 and CD8 levels in each group and analyzed the differences. Results: We found a significant difference in CD4 and CD8 levels in MTB and MDR TB conditions. Conclusion: This study shows that acute infection in experimental mice with MTB and MDR TB with or without diabetes had the highest levels of both CD4 and CD8 cells, which can be a sign of increased cellular immunity in a mice model.
AB - Background: Tuberculosis (TB) remains a major global health problem, in the top 10 causes of death. As a regulator of the immune response, T-helper (Th) cells activate other lymphocytes from the immune system, such as B cells, to destroy the TB pathogen by releasing CD4 and CD8 Th cells. Diabetes mellitus (DM) is a known cause of developing active pulmonary TB. Few studies have examined the biomolecular expression affecting Mycobacterium tuberculosis (MTB) and multidrug-resistant (MDR) MTB, which are associated with low immunity represented by TB in diabetes and CD4 and CD8 levels. Materials and methods: This animal study used a post-test control group design. We performed an experimental study using 30 BALB/c mice, each weighing 25 g. It included six experimental animal groups, of which three had a diabetes condition induced using intraperitoneal streptozotocin, and all were infected with MTB or MDR TB. We evaluated the CD4 and CD8 levels in each group and analyzed the differences. Results: We found a significant difference in CD4 and CD8 levels in MTB and MDR TB conditions. Conclusion: This study shows that acute infection in experimental mice with MTB and MDR TB with or without diabetes had the highest levels of both CD4 and CD8 cells, which can be a sign of increased cellular immunity in a mice model.
KW - CD4
KW - CD8
KW - Diabetes mellitus
KW - Multidrug resistance
KW - Mycobacterium tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=85111697764&partnerID=8YFLogxK
U2 - 10.1016/j.amsu.2021.102596
DO - 10.1016/j.amsu.2021.102596
M3 - Article
AN - SCOPUS:85111697764
SN - 2049-0801
VL - 68
JO - Annals of Medicine and Surgery
JF - Annals of Medicine and Surgery
M1 - 102596
ER -
