@inproceedings{1b07ea8c22eb46abb6f267b9be7b917f,
title = "Analysis of α-terthienyl (2,2′:5′,2″- terthiophene) in Indonesian Tagetes erecta flower by HPLC and LC-MS/MS",
abstract = "Tagetes erecta flowers contain nematoside compound α-Terthienyl (2,2′:5′,2″-Terthiophene) which are toxic to erythrocytes and human skin. In this study, analysis of the content of α-Terthienyl (2,2′:5′,2″-Terthiophene) in orange and yellow Tagetes erecta flowers originating from several areas in West Java by HPLC and LC-MS/MS have been studied. The flowers are dried in an oven at 45 °C, then crushed in a blender and sieved with a 0.35 μm sieve. The flower powder is then extracted with n-hexane using a waterbath shaker at 65 °C for 1 h. The solvent extraction then evaporated in a water bath until dry. The residue in the form of a thick extract is dissolved with ethanol, then filtered with a 0.45 μm membrane filter, then injected into the HPLC system. This filtered solution was also diluted 20 times with acetonitrile for analysis by LC-MS/MS. Content of α-Terthienyl (2,2′:5′,2″-Terthiophene) obtained from the HPLC and LC-MS/MS analysis were not significantly different. The α-Terthienyl (2,2′:5′,2″-Terthiophene) peak is well separated from the other peaks. Orange flowers from each region contain higher α-Terthienyl (2,2′:5′,2″-Terthiophene) than yellow flowers. This method is sensitive and easy to do because it does not require further purification of the extract and uses a simple mobile phase.",
keywords = "HPLC, LC-MS/MS, Tagetes erecta, waterbath shaker, α-Terthienyl (2,2':5',2''-Terthiophene)",
author = "Zannah, {N. S.} and Cahyana, {A. H.} and A. Saefumillah",
note = "Publisher Copyright: {\textcopyright} 2021 Author(s).; 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020 ; Conference date: 27-10-2020 Through 28-10-2020",
year = "2021",
month = jul,
day = "23",
doi = "10.1063/5.0059813",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Ivandini, {Tribidasari A.} and Churchill, {David G.} and Youngil Lee and Alias, {Yatimah Binti} and Chris Margules",
booktitle = "Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020",
address = "United States",
}