TY - GEN
T1 - Analysis of α-terthienyl (2,2′:5′,2″- terthiophene) in Indonesian Tagetes erecta flower by HPLC and LC-MS/MS
AU - Zannah, N. S.
AU - Cahyana, A. H.
AU - Saefumillah, A.
N1 - Publisher Copyright:
© 2021 Author(s).
PY - 2021/7/23
Y1 - 2021/7/23
N2 - Tagetes erecta flowers contain nematoside compound α-Terthienyl (2,2′:5′,2″-Terthiophene) which are toxic to erythrocytes and human skin. In this study, analysis of the content of α-Terthienyl (2,2′:5′,2″-Terthiophene) in orange and yellow Tagetes erecta flowers originating from several areas in West Java by HPLC and LC-MS/MS have been studied. The flowers are dried in an oven at 45 °C, then crushed in a blender and sieved with a 0.35 μm sieve. The flower powder is then extracted with n-hexane using a waterbath shaker at 65 °C for 1 h. The solvent extraction then evaporated in a water bath until dry. The residue in the form of a thick extract is dissolved with ethanol, then filtered with a 0.45 μm membrane filter, then injected into the HPLC system. This filtered solution was also diluted 20 times with acetonitrile for analysis by LC-MS/MS. Content of α-Terthienyl (2,2′:5′,2″-Terthiophene) obtained from the HPLC and LC-MS/MS analysis were not significantly different. The α-Terthienyl (2,2′:5′,2″-Terthiophene) peak is well separated from the other peaks. Orange flowers from each region contain higher α-Terthienyl (2,2′:5′,2″-Terthiophene) than yellow flowers. This method is sensitive and easy to do because it does not require further purification of the extract and uses a simple mobile phase.
AB - Tagetes erecta flowers contain nematoside compound α-Terthienyl (2,2′:5′,2″-Terthiophene) which are toxic to erythrocytes and human skin. In this study, analysis of the content of α-Terthienyl (2,2′:5′,2″-Terthiophene) in orange and yellow Tagetes erecta flowers originating from several areas in West Java by HPLC and LC-MS/MS have been studied. The flowers are dried in an oven at 45 °C, then crushed in a blender and sieved with a 0.35 μm sieve. The flower powder is then extracted with n-hexane using a waterbath shaker at 65 °C for 1 h. The solvent extraction then evaporated in a water bath until dry. The residue in the form of a thick extract is dissolved with ethanol, then filtered with a 0.45 μm membrane filter, then injected into the HPLC system. This filtered solution was also diluted 20 times with acetonitrile for analysis by LC-MS/MS. Content of α-Terthienyl (2,2′:5′,2″-Terthiophene) obtained from the HPLC and LC-MS/MS analysis were not significantly different. The α-Terthienyl (2,2′:5′,2″-Terthiophene) peak is well separated from the other peaks. Orange flowers from each region contain higher α-Terthienyl (2,2′:5′,2″-Terthiophene) than yellow flowers. This method is sensitive and easy to do because it does not require further purification of the extract and uses a simple mobile phase.
KW - HPLC
KW - LC-MS/MS
KW - Tagetes erecta
KW - waterbath shaker
KW - α-Terthienyl (2,2':5',2''-Terthiophene)
UR - http://www.scopus.com/inward/record.url?scp=85112060412&partnerID=8YFLogxK
U2 - 10.1063/5.0059813
DO - 10.1063/5.0059813
M3 - Conference contribution
AN - SCOPUS:85112060412
T3 - AIP Conference Proceedings
BT - Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020
A2 - Ivandini, Tribidasari A.
A2 - Churchill, David G.
A2 - Lee, Youngil
A2 - Alias, Yatimah Binti
A2 - Margules, Chris
PB - American Institute of Physics Inc.
T2 - 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020
Y2 - 27 October 2020 through 28 October 2020
ER -