In improving the quality management system, the testing laboratory uses the ISO/IEC 17025 standard as a benchmark for the general requirements of testing/calibration competence, including sampling. This standard is also used for quality, administrative and technical activities. Customer laboratories, regulators and accreditation bodies may also use them to confirm or acknowledge the competence of the laboratory. The application of standards in laboratory testing is closely related to achieving the quality objectives contained in the laboratory. By using the standard ISO/IEC 17025 then the resulting product is guaranteed security, reliable and certainly has a quality that no doubt. Testing laboratory can be said to run effectively when it can manage or manage flexibility, customer, production, value-oriented, and the main job of its employees. Therefore, the analysis of the effectiveness of ISO/IEC 17025 implementation takes an important role for the testing laboratory. The Focus Group Discussion (FGD) method consisting of several competent assessors is instrumental in assessing any clauses related to the effectiveness of ISO implementation.