This research was conducted to study ergonomic aspect from University of Indonesia bicycle in virtual environment. Software Jack 6.0 was used to analyze it. PEI was used as approach that integrated the results of three methods: Lower Back Analysis, Ovako Working Posture Analysis, and Rapid Upper Limb Assessment. The research objective is to evaluate existing design of University of Indonesia bicycle and to determine the most ergonomic redesign which concern with handlebar height and saddle height modification. The result showed that the most ergonomic design of University of Indonesia bicycle is the one with the highest handlebar height (22 cm) and the lowest saddle height (11 cm).