Pain is an effect that is produced in a conscious state when the stimulus reaches the brain, where stimulation comes from nerve impulses produced by dangerous stimuli. Analgesic medication plays an important role in reducing pain, so the severe effect from long-term pain can be reduced, such as lower mind concentration and nausea. Indonesian anti-neurodegenerative mixed-herb formula consists of cloves (Syzygium aromaticum), ginger (Zingiber officinale), and nutmeg (Myristica fragrans) is originated from Central Java, Indonesia and has been used as a traditional medication for reducing neural pain. In this study, the formula was extracted with water and was tested for its analgesic activity in white-strain mice (Mus musculus). The extraction process was conducted with 10 g of dried sample in 250 ml of water, using the reflux method at a temperature of 85°C for 90 minutes. The analgesic test was conducted with five groups: control, positive, paracetamol, and three dosage groups. The test used the acetic-induced Writhing test method, and each mouse was measured its total number of stretching. The result has shown that paracetamol effectively reduced pain after 10 minutes from oral medication for 80.88% effectiveness, while the formula's extracts needed 25 minutes to reduce the pain with the dosage 3 group gave the biggest effectiveness 21.95%. The ANOVA single factor was conducted with a 0.01 significance level, resulting in a significant difference. Thus, it can be concluded that the Indonesian anti-neurodegenerative mixed-herb water extract has analgesic activity.