An analysis of hydrocarbon degrading capabilities from three bacterial isolates from mangrove habitats in Muara Angke Jakarta

Sitaresmi, Yasman, A. Zahra, H. S. Putri, T. I. Hardiyanti

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Bacteria have been widely used as an agent for bioremediation of hydrocarbon contamination. Exploration of mangrove habitats for potential bacterial isolates have also been carried out. This study aims to explore the potential of three bacterial isolates from mangrove habitats in Jakarta for hydrocarbon degrading capabilities. Screening for potential bacterial isolates was carried out according to Ilyina (2003). Bacterial growth was measured using the Total Plate Count method and selected isolates were then analyzed for hydrocarbon degrading capabilities using Gas Chromatography. From 12 (twelve) soil samples, 11 (eleven) isolates were further analyzed and 3 (three) isolates: HL 8-5, SM 2-2 and SM 1-7 displayed good growth in Bushnell Haas medium containing 1% diesel oil. Analysis of remaining hydrocarbon with Gas Chromatography showed a decrease in diesel oil concentration, which was indicated by a decrease in concentration of several alkane hydrocarbons contained in diesel oil with a C15-C24 chain length. The percentage of hydrocarbon decrease varies among the three strains. Strain HL 8-5 displayed the ability to reduce Tricosane and Tetracosane at 4,94% and 9,43% respectively. Strain SM 1-7 displayed the ability to reduce eicosane, a C20 alkane hydrocarbon at 19,14% and heptadecane, a C17 alkane hydrocarbon at 20,17% while strain SM 2-2 displayed a decrease of heptadecane 20,17%. The results indicate that each strain has a different type of enzyme for utilizing and degrading hydrocarbons. Further work is recommended for the strain SM 1-7 as phenotypic characterization suggests the strain belong in the genus Pseudomonas, which is known to have hydrocarbon degrading capabilities.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

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