Amylase activity of actinomycetes isolated from mangrove litter at Pramuka Island, Kepulauan Seribu, DKI Jakarta, Indonesia

E. L. Fasa, I. Santoso, Y. Yasman, A. E. Maryanto, Q. G. Fadhilah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Research about amylase activity of 28 isolates of actinomycetes isolated from mangrove litter at Pramuka Island, Kepulauan Seribu, DKI Jakarta, Indonesia, has been carried out. Amylase activity was screened using the iodine method with starch agar containing 0.1 % soluble starch, then expressed as amylase Activity Index (AI). Among the tested isolates, SM 25 was the most potential isolate for amylase activity with AI=3.21 which was further tested. Amylase activity of isolate SM 25 was measured using the dinitrosalicylic acid (DNS) method with starch broth 2 % containing soluble starch or rice flour. The concentration of reducing sugar in the growth medium was also measured after 4 days incubation. The result showed that isolate SM 25 has higher amylase activity in medium with soluble starch (5.02 U/mL) than rice flour (3.33 U/mL). However, the concentration of reducing sugar was higher in medium with rice flour (582.42 μg/mL) than soluble starch (407.40 μg/mL).

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
Country/TerritoryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Actinomycetes
  • amylase activity
  • DNS method

Fingerprint

Dive into the research topics of 'Amylase activity of actinomycetes isolated from mangrove litter at Pramuka Island, Kepulauan Seribu, DKI Jakarta, Indonesia'. Together they form a unique fingerprint.

Cite this