Introduction: Mesenchymal stem cell (MSC)-derived conditioned medium (CM) has recently garnered worldwide interests due to the growth factors contained within. The objective of this study was to identify the quantity of bone morphogenetic protein-2 (BMP-2), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) in adipose tissue MSC (AT-MSC), umbilical cord MSC (UC-MSC) secretome and bone marrow MSC (BM-MSC) in-vitro. Methods: Secretome samples from BM-MSCs, AT-MSCs and UC-MSCs were grouped based on the presence/absence of serum in culture medium and harvesting time and were analyzed using Human BMP-2, VEGF, and EGF ELISA Kits. This study identified whether BMP-2 was present in the secretome of all three MSCs sources and measured the levels of BMP-2, EGF and VEGF in the secretome. Results: The distribution of value for BMP-2 was centered around zero in all sources of MSC secretome. The levels of EGF and VEGF were significantly different between different donor samples for AT-MSC secretome (p < 0.009 and p < 0.005). The level of VEGF in AT-MSC and UC-MSC secretomes were approximately 1473.5 pg/mL and 0 pg/mL, respectively. In AT-MSC secretome, the level of EGF was found decreased with increasing passage (p < 0.009), while the level of VEGF was found increased with increasing passage (p < 0.001). Conclusions: The level of BMP-2 was found to be really low in any MSC secretome sources. Different donor samples gave a significantly different level of EGF and VEGF in AT-MSC secretome, but not for UC-MSC secretome. The level of VEGF was found high in AT-MSC secretome and was constantly high regardless of the timing of secretome collection.
|Number of pages||10|
|Journal||Journal of Stem Cells|
|Publication status||Published - 2020|
- Mesenchymal stem cell (MSC)