TY - JOUR
T1 - Aminolevulinic Acid Dehydratase Allelic Frequency and LeadToxicity in Children Under-Five in a Former Used Lead-AcidBattery Area
AU - Irawati, Yana
AU - Kusnoputranto, Haryoto
AU - Achmadi, Umar Fahmi
AU - Safrudin, Ahmad
AU - Sitorus, Alfred
AU - Risandi, Rifqi
AU - Wangsamuda, Suradi
AU - Permana, Dendi Hadi
AU - Syahrani, Lepa
AU - Dewayanti, Farahana Kresno
AU - Asih, Puji B.S.
AU - Syafruddin, Din
N1 - Funding Information:
The authors would like to thank the people of Cinangka Village, Bogor District, who voluntarily joined the study as respondents. Also the authors thank to the village health workers, village officials (Nurdin, Irfan Lahardi), and staff of the Primary Health Care Pasir Orai (Rosa Ria, Linda) who facilitated the implementation of this study. The authors also thank Sandy, Nurul, Fitri, Agus, Amel, and Agung from Komite Penghapusan Bensin Bertimbal (KPBB) for their assistance. This study was supported by the Eijkman Institute for Molecular Biology, which is part of the Ministry of Research and Technology/National Agency for Research and Innovation (RISTEK-BRIN), and financing from the Ministry of Finance’s Educational Fund Management Institution, Lembaga Pengelola Dana Pendidikan, abbreviated LPDP (PRJ-26/LPDP.4/2020).
Publisher Copyright:
© 2022 Universitas Indonesia, Faculty of public health. All Rights Reserved.
PY - 2022/2
Y1 - 2022/2
N2 - Polymorphisms in the Aminolevulinic Acid Dehydratase(ALAD) gene responsible for the ALAD1and ALAD2alleles have been implicated in susceptibility tolead toxicity. This study aimed to determine the allelic frequency of ALAD2among children living in Bogor District, Indonesia, and its association with bloodlead levels (BLLs) and lead toxicity. A cross-sectional study involving 128 children was conducted during September-October 2019 in the former ULAB areain Cinangka Village. The ALADpolymorphism, BLLs, and hematological parameters were evaluated. Blood samples were taken for dried blood spotting onfilter paper, blood film, and BLL measurement. The PCR amplification and sequencing of the genomic DNA revealed the presence of two forms of the ALAD2allele: 177C and 177T with a frequency of 0.05. Analysis of the correlation between the ALAD2allele, BLLs, and basophilic stippling revealed that ALAD2carriers had a five times higher risk of high BLLs, (OR = 5.359, p-value = 0.155) and had a slightly higher risk of exhibiting basophilic stippling (OR = 1.09, p-value = 1.000). Although not statistically significant, these findings suggested that the ALADgenotype may modify BLLs and lead to toxicity. The ALAD2allele(177T) is firstly reported in any population in the world.
AB - Polymorphisms in the Aminolevulinic Acid Dehydratase(ALAD) gene responsible for the ALAD1and ALAD2alleles have been implicated in susceptibility tolead toxicity. This study aimed to determine the allelic frequency of ALAD2among children living in Bogor District, Indonesia, and its association with bloodlead levels (BLLs) and lead toxicity. A cross-sectional study involving 128 children was conducted during September-October 2019 in the former ULAB areain Cinangka Village. The ALADpolymorphism, BLLs, and hematological parameters were evaluated. Blood samples were taken for dried blood spotting onfilter paper, blood film, and BLL measurement. The PCR amplification and sequencing of the genomic DNA revealed the presence of two forms of the ALAD2allele: 177C and 177T with a frequency of 0.05. Analysis of the correlation between the ALAD2allele, BLLs, and basophilic stippling revealed that ALAD2carriers had a five times higher risk of high BLLs, (OR = 5.359, p-value = 0.155) and had a slightly higher risk of exhibiting basophilic stippling (OR = 1.09, p-value = 1.000). Although not statistically significant, these findings suggested that the ALADgenotype may modify BLLs and lead to toxicity. The ALAD2allele(177T) is firstly reported in any population in the world.
KW - Aminolevulinic acid dehydratase-2allele
KW - Basophilic stippling
KW - Blood lead level
KW - Lead toxicity
UR - http://www.scopus.com/inward/record.url?scp=85125854329&partnerID=8YFLogxK
U2 - 10.21109/kesmas.v17i1.5525
DO - 10.21109/kesmas.v17i1.5525
M3 - Article
AN - SCOPUS:85125854329
SN - 1907-7505
VL - 17
SP - 66
EP - 73
JO - Kesmas
JF - Kesmas
IS - 1
ER -
