Amino Acid Sequence Differences of Cytotoxic T-Cell and Helper T-Cell Epitope of Wild-Type Measles Virus and Vaccine Virus in Indonesia

Made Setiawan, Agus Sjahrurachman, R. Fera Ibrahim, Agus Suwandono

Research output: Contribution to journalArticlepeer-review

Abstract

Measles virus is a negative single-strand RNA virus, a member of genus Morbillivirus which belongs to Paramyxoviridae family. RNA is packed by nucleokapsid (N) protein. Nucleoprotein is very important for RNA replication and translation. Moreover, nucleoprotein is involved in inducing the protective antibody in the hosts. When the differences amino acid of cytotoxic T-Cell and helper T-Cell occurs in nucleoprotein, leadding to the differences of antibody specificity to those epitope. The purpose of this research is to know the amino acid sequence differences of cytotoxic T-cell and Helper T-Cell epitope of nucleoprotein between the wild-type measles virus and the measles vaccine virus. This research is a laboratory-based research using biomoleculer and bioinformatic technology to analyse the virus gene. We found the amino acid sequence differences of cytotoxic T-cell epitope of N protein (52-59) between the wild-type measles virus and the CAM-70 vaccine virus. However, we did not find the differences between the wildtype measles virus with Schwarz vaccine and Edmonston-wt. On the other hand, we found the differences of Helper T-Cell epitope of N protein between the wild-type measles virus and CAM-70 vaccine virus, and between Schwarz vaccine virus and Edmonston-wt. The study concluded that there are amino acid sequence differences of B-cell epitope and T-cell epitope of N protein between the wild-type measles virus and the vaccine virus.
Original languageEnglish
JournalJournal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Publication statusPublished - 2009

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