Alpha-glucosidase inhibitory activity of stingless bee honey from Tetragonula biroi and Tetragonula laeviceps

Oktavia Rahmawati, Diah Kartika Pratami, Rika Raffiudin, Muhamad Sahlan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Citations (Scopus)

Abstract

Diabetes Mellitus (DM) is a metabolic disease characterized by chronic hyperglycemia in the presence of carbohydrate, fat, and protein metabolism disorder. Diabetes mellitus can be treated by inhibiting the activity of the alpha-glucosidase enzyme that serves to break down carbohydrates into glucose. Honey is known to have many health benefits. This study aims to determine the alpha-glucosidase enzyme inhibitory activity of stingless bee honey from Tetragonula biroi and Tetragonula laeviceps. Stingless bee honey from T. biroi and T. laeviceps with various concentrations (50-1000ppm) were tested to obtain the IC 50 value. Acarbose was used as a positive control. This study was performed by measuring the absorbance of the reaction mixture (phosphate buffer, 4-nitrophenyl α-D-glucopyranoside, α-glucosidase, and sample) using a microplate reader at 410nm. The results obtained that stingless bee honey from T. biroi and T. laeviceps has a potency to inhibit the activity of the α-glucosidase enzyme. However, the methanol extract of T. laeviceps honey has the highest ability to inhibit the activity of the α-glucosidase enzyme with IC 50 values of around 2394.69ppm. Therefore, some animal testing or more research were needed to investigate the potential of stingless bee honey from T. biroi and T. laeviceps for diabetic treatment.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • Alpha-glucosidase
  • diabetes mellitus
  • stingless bee honey
  • Tetragonula biroi
  • Tetragonula laeviceps

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