Alpha-glucosidase inhibitory activity of Garcinia lateriflora Blume Leaves

Garcinia lateriflora Blume., that belongs to the genus Garcinia, is known to contain polyphenol compounds that are likely to inhibit alpha-glucosidase in the treatment of diabetes mellitus. Therefore, this study aimed to evaluate and acquire the most biologically active fraction from leaves of G. lateriflora in inhibiting alpha-glucosidase. The separation of the active fraction was conducted using column chromatography and identified by thin layer chromatography. Alpha-glucosidase inhibitory activity test carried out in vitro using spectrophotometric method with p-nitrophenyl-α-D-glucopiranoside as the substrate. 17 and 12 fractions were obtained from ethyl acetate and methanol extracts, respectively. Fraction 13 (EA13) of ethyl acetate extract and fraction 10 (Me10) of methanol extract were showed the highest percent inhibition compared with the other fraction with IC₅₀ values 8.96 μg/ml and 18.52 μg/ml, respectively. While the IC₅₀ value for Acarbose is 39.53 μg/ml. Furthermore, fraction EA13 is the most active fraction in inhibiting alpha-glucosidase compared with the extract, other fractions, and Acarbose.