TY - JOUR
T1 - Alpha-1-acid glycoprotein as potential biomarker for alpha-fetoprotein-low hepatocellular carcinoma
AU - Bachtiar, Indra
AU - Kheng, Valentine
AU - Wibowo, Gunawan A.
AU - Gani, Rino A.
AU - Hasan, Irsan
AU - Sanityoso, Andri
AU - Budhihusodo, Unggul
AU - Lelosutan, Syafruddin A.R.
AU - Martamala, Ruswhandi
AU - Achwan, Wenny A.
AU - Soemoharjo, Soewignyo
AU - Sulaiman, Ali
AU - Lesmana, Laurentius M. Adrianto
AU - Tai, Susan
N1 - Funding Information:
The authors appreciate Prof. David Vaux (La Trobe University, Australia) and Dr. Juandy Jo (Uni-Freiburg, Germany) for critical reading. We thank Rama Dhenni, Griskalia Christine, and Shinta Soraya for sample collection and preparation. This work was supported by Mochtar Riady Institute for Nanotechnology (MRIN) Funding (Budget no. cc042/2007/08).
PY - 2010
Y1 - 2010
N2 - Background. The outcome of patients with hepatocellular carcinoma (HCC) remains poor because of late diagnosis. We determined the performances of α-1-acid glycoprotein (AAG) and des-γ-carboxy prothrombin (DCP) for the diagnosis of HCC, especially for α-fetoprotein (AFP)-low HCC. Methods. Of the 220 patients included in this retrospective study, 124 had HCC, and 61 (49%) of these were AFP-low HCC (AFP ≤ 20 ng/mL). The remaining 96 patients, including 49 with chronic hepatitis B or C and 47 with cirrhosis, were considered as control. Plasma AAG was analyzed using high performance liquid chromatography (HPLC) and confirmed using Western blot technique. Results. When all patients with HCC were evaluated, the area under receiver operating characteristic (ROC) curves for AAG (0.94, 95% CI: 0.91-0.97) and DCP (0.92, 95% CI: 0.88-0.95) were similar (P = 0.40). AAG had better area under ROC curve (0.96, 95% CI: 0.94-0.99) than DCP (0.87, 95% CI: 0.81-0.93) for AFP-low HCC (P < 0.05). At the specificity 95%, the sensitivity of AAG was higher in AFP-low HCC than in AFP-high HCC (82% and 62%, respectively). In contrast, higher sensitivity was obtained from DCP in discriminating HCC patients with low AFP than that in high AFP (57% and 90%, respectively). Conclusion. Our cross-sectional study showed that AAG was better performance in diagnosing HCC patients with low AFP, while DCP did better in those with high AFP.
AB - Background. The outcome of patients with hepatocellular carcinoma (HCC) remains poor because of late diagnosis. We determined the performances of α-1-acid glycoprotein (AAG) and des-γ-carboxy prothrombin (DCP) for the diagnosis of HCC, especially for α-fetoprotein (AFP)-low HCC. Methods. Of the 220 patients included in this retrospective study, 124 had HCC, and 61 (49%) of these were AFP-low HCC (AFP ≤ 20 ng/mL). The remaining 96 patients, including 49 with chronic hepatitis B or C and 47 with cirrhosis, were considered as control. Plasma AAG was analyzed using high performance liquid chromatography (HPLC) and confirmed using Western blot technique. Results. When all patients with HCC were evaluated, the area under receiver operating characteristic (ROC) curves for AAG (0.94, 95% CI: 0.91-0.97) and DCP (0.92, 95% CI: 0.88-0.95) were similar (P = 0.40). AAG had better area under ROC curve (0.96, 95% CI: 0.94-0.99) than DCP (0.87, 95% CI: 0.81-0.93) for AFP-low HCC (P < 0.05). At the specificity 95%, the sensitivity of AAG was higher in AFP-low HCC than in AFP-high HCC (82% and 62%, respectively). In contrast, higher sensitivity was obtained from DCP in discriminating HCC patients with low AFP than that in high AFP (57% and 90%, respectively). Conclusion. Our cross-sectional study showed that AAG was better performance in diagnosing HCC patients with low AFP, while DCP did better in those with high AFP.
UR - http://www.scopus.com/inward/record.url?scp=78549285667&partnerID=8YFLogxK
U2 - 10.1186/1756-0500-3-319
DO - 10.1186/1756-0500-3-319
M3 - Article
C2 - 21092242
AN - SCOPUS:78549285667
SN - 1756-0500
VL - 3
JO - BMC Research Notes
JF - BMC Research Notes
M1 - 319
ER -
