TY - JOUR
T1 - ALP gene expression in cDNA samples from bone tissue engineering using a HA/TCP/Chitosan scaffold
AU - Stephanie, N.
AU - Katarina, H.
AU - Amir, L. R.
AU - Gunawan, H. A.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2017/8/30
Y1 - 2017/8/30
N2 - This study examined the potential use of hydroxyapatite (HA)/tricalcium phosphate (TCP)/Chitosan as a bone tissue engineering scaffold. The potential for using HA/TCP/chitosan as a scaffold was analyzed by measuring expression of the ALP osteogenic gene in cDNA from bone biopsies from four Macaque nemestrina. Experimental conditions included control (untreated), treatment with HA/TCP 70:30, HA/TCP 50:50, and HA/TCP/chitosan. cDNA samples were measured quantitively with Real-Time PCR (qPCR) and semi-quantitively by gel electrophoresis. There were no significant differences in ALP gene expression between treatment subjects after two weeks, but the HA/TCP/chitosan treatment gave the highest level of expression after four weeks. The scaffold using the HA/TCP/chitosan combination induced a higher level of expression of the osteogenic gene ALP than did scaffold without chitosan.
AB - This study examined the potential use of hydroxyapatite (HA)/tricalcium phosphate (TCP)/Chitosan as a bone tissue engineering scaffold. The potential for using HA/TCP/chitosan as a scaffold was analyzed by measuring expression of the ALP osteogenic gene in cDNA from bone biopsies from four Macaque nemestrina. Experimental conditions included control (untreated), treatment with HA/TCP 70:30, HA/TCP 50:50, and HA/TCP/chitosan. cDNA samples were measured quantitively with Real-Time PCR (qPCR) and semi-quantitively by gel electrophoresis. There were no significant differences in ALP gene expression between treatment subjects after two weeks, but the HA/TCP/chitosan treatment gave the highest level of expression after four weeks. The scaffold using the HA/TCP/chitosan combination induced a higher level of expression of the osteogenic gene ALP than did scaffold without chitosan.
UR - http://www.scopus.com/inward/record.url?scp=85029606493&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/884/1/012112
DO - 10.1088/1742-6596/884/1/012112
M3 - Conference article
AN - SCOPUS:85029606493
SN - 1742-6588
VL - 884
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012112
T2 - 1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017
Y2 - 15 July 2017 through 16 July 2017
ER -