ALP gene expression in cDNA samples from bone tissue engineering using a HA/TCP/Chitosan scaffold

N. Stephanie, H. Katarina, Lisa Rinanda Amir, Harun Asjiq Gunawan

Research output: Contribution to journalConference articlepeer-review

2 Citations (Scopus)

Abstract

This study examined the potential use of hydroxyapatite (HA)/tricalcium phosphate (TCP)/Chitosan as a bone tissue engineering scaffold. The potential for using HA/TCP/chitosan as a scaffold was analyzed by measuring expression of the ALP osteogenic gene in cDNA from bone biopsies from four Macaque nemestrina. Experimental conditions included control (untreated), treatment with HA/TCP 70:30, HA/TCP 50:50, and HA/TCP/chitosan. cDNA samples were measured quantitively with Real-Time PCR (qPCR) and semi-quantitively by gel electrophoresis. There were no significant differences in ALP gene expression between treatment subjects after two weeks, but the HA/TCP/chitosan treatment gave the highest level of expression after four weeks. The scaffold using the HA/TCP/chitosan combination induced a higher level of expression of the osteogenic gene ALP than did scaffold without chitosan.

Original languageEnglish
Article number012112
JournalJournal of Physics: Conference Series
Volume884
Issue number1
DOIs
Publication statusPublished - 30 Aug 2017
Event1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017 - Depok, West Java, Indonesia
Duration: 15 Jul 201716 Jul 2017

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