TY - GEN
T1 - Allelic pattern and diversity in wild stocks of the giant freshwater prawn (Macrobrachium rosenbergii) from Indonesia
AU - Maryanto, Andi Eko
AU - Binur, Robi
AU - Pancoro, Adi
N1 - Publisher Copyright:
© 2016 Author(s).
PY - 2016/4/19
Y1 - 2016/4/19
N2 - In order to provide basic information for restocking program, allelic pattern and diversity in wild stocks of the giant freshwater prawn (Macrobrachium rosenbergii) from Indonesia was studied using microsatellite analysis. The research was performed among the population from nine wildtype populations, Medan (MDN), Padang (PDG), Jambi (JMB), Lampung (LPG), Palembang (PLG), Ciamis (CMS), Palangkaraya (PLK), Samarinda (SMD), and Papua (PPA). Polymerase chain reaction (PCR) with fluorescent label detection showed that a total of 38 alleles were amplified from five primers. The average number of alleles (Na) per locus, observed heterozygosity (Ho), expected heterozygosity (He) and Polymorphic Information Content (PIC) were 11.2, 0.902, 0.664, and 0.666, respectively. The result of the present study of the giant freshwater prawn genetic diversty supports the basic information for broodstock restocking program. On other hand, the wildtype populations from Papua and Palembang displayed the highest genetic diversity and will be preferably used for future selective breeding.
AB - In order to provide basic information for restocking program, allelic pattern and diversity in wild stocks of the giant freshwater prawn (Macrobrachium rosenbergii) from Indonesia was studied using microsatellite analysis. The research was performed among the population from nine wildtype populations, Medan (MDN), Padang (PDG), Jambi (JMB), Lampung (LPG), Palembang (PLG), Ciamis (CMS), Palangkaraya (PLK), Samarinda (SMD), and Papua (PPA). Polymerase chain reaction (PCR) with fluorescent label detection showed that a total of 38 alleles were amplified from five primers. The average number of alleles (Na) per locus, observed heterozygosity (Ho), expected heterozygosity (He) and Polymorphic Information Content (PIC) were 11.2, 0.902, 0.664, and 0.666, respectively. The result of the present study of the giant freshwater prawn genetic diversty supports the basic information for broodstock restocking program. On other hand, the wildtype populations from Papua and Palembang displayed the highest genetic diversity and will be preferably used for future selective breeding.
UR - http://www.scopus.com/inward/record.url?scp=84984534174&partnerID=8YFLogxK
U2 - 10.1063/1.4946973
DO - 10.1063/1.4946973
M3 - Conference contribution
AN - SCOPUS:84984534174
T3 - AIP Conference Proceedings
BT - International Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
A2 - Mart, Terry
A2 - Triyono, Djoko
PB - American Institute of Physics Inc.
T2 - 1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
Y2 - 3 November 2015 through 4 November 2015
ER -