Alginate-chitosan microencapsulated cells for improving cd34+ progenitor maintenance and expansion

Retno Wahyu Nurhayati, Rafianto Dwi Cahyo, Gita Pratama, Dian Anggraini, Wildan Mubarok, Mime Kobayashi, Radiana Dhewayani Antarianto

Research output: Contribution to journalArticlepeer-review

Abstract

Protocols for isolation, characterization, and transplantation of hematopoietic stem cells (HSCs) have been well established. However, difficulty in finding human leucocyte antigens (HLA)-matched donors and scarcity of HSCs are still the major obstacles of allogeneic transplanta-tion. In this study, we developed a double-layered microcapsule to deliver paracrine factors from non-matched or low-matched HSCs to other cells. The umbilical cord blood-derived hematopoietic progenitor cells, identified as CD34+ cells, were entrapped in alginate polymer and further protected by chitosan coating. The microcapsules showed no toxicity for surrounding CD34+ cells. When CD34+ cells-loaded microcapsules were co-cultured with bare CD34+ cells that have been collected from unrelated donors, the microcapsules affected surrounding cells and increased the percentage of CD34+ cell population. This study is the first to report the potency of alginate-chitosan microcap-sules containing non-HLA-matched cells for improving proliferation and progenitor maintenance of CD34+ cells.

Original languageEnglish
Article number7887
JournalApplied Sciences (Switzerland)
Volume11
Issue number17
DOIs
Publication statusPublished - 1 Sep 2021

Keywords

  • Alginate
  • CD34
  • Chitosan
  • Hematopoietic
  • Megakaryocyte
  • Microencapsulation
  • Progenitor
  • Proliferation
  • Stem cells

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