Alendronate effect in RANKL concentration at ovariectomized Sprague Dawley rat

Marzella Mega Lestari, Dwi Nugroho Juanda, Dewi Fatma Suniarti, Dondin Sajuthi

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In this study, we analyze the relationship between alendronate concentration and serum-RANKL concentration 24 Sprague Dawley female rats that had given birth, aged 5 months were performed bilateral ovariectomy. Rats were divided into four groups. Groups A, B, C were treated with subcutaneous injection 4 times per week with alendronate 0.08 ml, 0.29 ml, 0.58 ml. Group O were not given alendronate, and were categorized as control group. The RANKL concentration were measured quantitatively with Enzyme-linked Immunosorbent Assays (ELISA) via blood sampling that were taken at before ovariectomy (day 0), prior to implantation (day 15), and intracardially after euthanasia (day 70). As the result from study above, high dosage of alendronate there was no decrease in RANKL concentration, but there was a significant change in RANKL concentration. thus the hypothesis that the higher the dose of alendronate the higher the decrease of RANKL cannot be accepted There is a relationship between high dosage of alendronate with the change in RANKL concentrations. The greatest RANKL changes were at concentrations of 35 mg (0.29 ml) and 70 mg (0.58 ml).

Original languageEnglish
Pages (from-to)846-849
Number of pages4
JournalJournal of International Dental and Medical Research
Issue number3
Publication statusPublished - 1 Jan 2018


  • Alendronate
  • Implant
  • Osteoporosis
  • RANKL concentration


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