As a process that relates to culture, acculturation becomes one important factor in tourism. Usually, the hosts borrow the result of acculturation in tourism activity more than the tourists (guests). Acculturation in tourism does not only occur in tourism practices today but also in the past, especially in colonial Indonesia. This article discusses acculturation in tourism activity in the Netherlands-Indies that became part of the tourism practices by using historical methods and Nunez’s concept of acculturation in tourism. By using guidebooks, newspapers, magazines, postcards, photographs, travelogues as sources, this article traces the result of acculturation at that time.The result shows that there was acculturation in tourism activity in the Netherlands-Indies. There were material objects and customs as tourism facilities, that could be seen, practised and enjoyed by the tourists. It concluded that at that time the tourists (the guests) borrowed the result of acculturation more from the hosts.