AI-2 of Aggregatibacter actinomycetemcomitans inhibits Candida albicans biofilm formation

Endang W. Bachtiar, Boy M. Bachtiar, Lucja M. Jarosz, Lisa R. Amir, Hari Sunarto, Hadas Ganin, Michael M. Meijler, Bastiaan P. Krom

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Aggregatibacter actinomycetemcomitans, a Gram-negative bacterium, and Candida albicans, a polymorphic fungus, are both commensals of the oral cavity but both are opportunistic pathogens that can cause oral diseases. A. actinomycetemcomitans produces a quorum-sensing molecule called autoinducer-2 (AI-2), synthesized by LuxS, that plays an important role in expression of virulence factors, in intra- but also in interspecies communication. The aim of this study was to investigate the role of AI-2 based signaling in the interactions between C. albicans and A. actinomycetemcomitans. A. actinomycetemcomitans adhered to C. albicans and inhibited biofilm formation by means of a molecule that was secreted during growth. C. albicans biofilm formation increased significantly when co-cultured with A. actinomycetemcomitans luxS, lacking AI-2 production. Addition of wild-type-derived spent medium or synthetic AI-2 to spent medium of the luxS strain, restored inhibition of C. albicans biofilm formation to wild-type levels. Addition of synthetic AI-2 significantly inhibited hypha formation of C. albicans possibly explaining the inhibition of biofilm formation. AI-2 of A. actinomycetemcomitans is synthesized by LuxS, accumulates during growth and inhibits C. albicans hypha- and biofilm formation. Identifying the molecular mechanisms underlying the interaction between bacteria and fungi may provide important insight into the balance within complex oral microbial communities.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalFrontiers in Cellular and Infection Microbiology
Volume4
Issue numberJUL
DOIs
Publication statusPublished - 2014

Keywords

  • Interspecies interaction
  • Oral microbiology
  • Quorum sensing

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