Aerobic Training Prevents Atherosclerosis Through Increased Production and Bioavailability of Nitric Oxide

Tyas Putri Utami, Ermita Isfandiary Ibrahim, Minarma Siagian, Ani Retno Prijanti

Research output: Contribution to journalArticlepeer-review


11 Apr 2019

Introduction: Atherosclerosis are related to endothelial dysfunction characterized by decreased nitric oxide (NO) production and bioavailability. Increased shear stress during aerobic exercise can increase endothelial nitric oxide synthase (eNOS) and antioxidant activity in the vascular wall. The aim of this study was to evaluate the effect of aerobic training on the production and bioavailability of nitric oxide in abdominal aorta tissues.Methods: Ten 9 week old male Wistar rats (150-200 grams) were randomly allocated into 2groups: training group and control group. Five times a week for 8 weeks, 20 minutes aerobic exercise with 90 seconds rest between 5 minutes run was conducted on a treadmill at a speed of 20 m/min. NO production, superoxide dismutase (SOD) specific activity, and malondialdehyde (MDA) in abdominal aorta tissues were measured using colorimetric (NO assay kit by BioVisionTM)and spectrophotometry (RANSOD kit by Randox LabTM) method.Results: Nitric oxide production of training group (0.0099?0.0074 mmol/g protein) was significantly (p=0.0045) higher than control group (0.0036?0,001 mmol/g protein). SOD specific activity of training group (0.2721?0.1183 U/mg protein) also significantly (p=0.0195) higher than control group (0.1361?0.0335 U/mg protein), whereas malondialdehyde of training group (0.0073?0.0018 nmol/mg tissue) slightly less than control (0.0079?0.0012 nmol/mg tissue)Conclusion: Aerobic training increased nitric oxide production and bioavailability by enhancing SOD specific activity and decreasing malondialdehyde in abdominal aorta tissues.
Original languageEnglish
JournalJournal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Publication statusPublished - 2016


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