Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation

Illmilda, Dini Asrianti, Anggraini Margono, Indah Julianto, Mettasari Puspa Wardoyo

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalJournal of International Dental and Medical Research
Volume12
Issue number2
Publication statusPublished - 1 Jan 2019

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Dental Pulp
Fibrin
Culture Media
Stem Cells
Blood Platelets
Cell Proliferation
Fibroblast Growth Factors
Flow Cytometry
Cell Culture Techniques
Control Groups

Keywords

  • Advanced platelet-rich fibrin (A-PRF)
  • Cultured medium
  • HDPSCs proliferation

Cite this

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title = "Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation",
abstract = "Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10{\%}, 20{\%}, and 25{\%} A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10{\%} and 20{\%} A-PRF, the proliferation of hDPSCs in 25{\%} A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25{\%} A-PRF on day 1 compared with 10{\%} and 20{\%} A-PRF.",
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Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation. / Illmilda, ; Asrianti, Dini; Margono, Anggraini; Julianto, Indah; Wardoyo, Mettasari Puspa.

In: Journal of International Dental and Medical Research, Vol. 12, No. 2, 01.01.2019, p. 396-400.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Illmilda,

AU - Asrianti, Dini

AU - Margono, Anggraini

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AU - Wardoyo, Mettasari Puspa

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