TY - JOUR
T1 - Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation
AU - Illmilda,
AU - Asrianti, Dini
AU - Margono, Anggraini
AU - Julianto, Indah
AU - Wardoyo, Mettasari Puspa
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
AB - Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
KW - Advanced platelet-rich fibrin (A-PRF)
KW - Cultured medium
KW - HDPSCs proliferation
UR - http://www.scopus.com/inward/record.url?scp=85069469190&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85069469190
VL - 12
SP - 396
EP - 400
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
SN - 1309-100X
IS - 2
ER -