TY - JOUR
T1 - Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation
AU - Illmilda,
AU - Asrianti, Dini
AU - Margono, Anggraini
AU - Julianto, Indah
AU - Wardoyo, Mettasari Puspa
N1 - Funding Information:
This research is approved and funded by HIBAH PITTA from Directorate Research and Community Engagement of the Universitas Indonesia. The authors also thank Mrs. Yanni Dirgantara, S.Si and Miss Emilia Rahmadaniah, S.Si from ProSTEM laboratory for their advice and support. The publication of this manuscript is supported by Universitas Indonesia.
Publisher Copyright:
© 2019 Journal of International Dental and Medical Research.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
AB - Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
KW - Advanced platelet-rich fibrin (A-PRF)
KW - Cultured medium
KW - HDPSCs proliferation
UR - http://www.scopus.com/inward/record.url?scp=85069469190&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85069469190
VL - 12
SP - 396
EP - 400
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
SN - 1309-100X
IS - 2
ER -