Abstract
Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
| Original language | English |
|---|---|
| Pages (from-to) | 396-400 |
| Number of pages | 5 |
| Journal | Journal of International Dental and Medical Research |
| Volume | 12 |
| Issue number | 2 |
| Publication status | Published - 1 Jan 2019 |
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Keywords
- Advanced platelet-rich fibrin (A-PRF)
- Cultured medium
- HDPSCs proliferation
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Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation. / Illmilda, ; Asrianti, Dini; Margono, Anggraini; Julianto, Indah; Wardoyo, Mettasari Puspa.
In: Journal of International Dental and Medical Research, Vol. 12, No. 2, 01.01.2019, p. 396-400.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Advanced Platelet Rich Fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation
AU - Illmilda,
AU - Asrianti, Dini
AU - Margono, Anggraini
AU - Julianto, Indah
AU - Wardoyo, Mettasari Puspa
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
AB - Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P < .05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P < .05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P > .05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF.
KW - Advanced platelet-rich fibrin (A-PRF)
KW - Cultured medium
KW - HDPSCs proliferation
UR - http://www.scopus.com/inward/record.url?scp=85069469190&partnerID=8YFLogxK
M3 - Article
VL - 12
SP - 396
EP - 400
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
SN - 1309-100X
IS - 2
ER -