TY - GEN
T1 - Administration of Centella asiatica ethanolic extract reduces tumor necrosis factor-alpha in hearts of aged sprague-dawley rats but not kidneys
AU - Hartanto, Jonathan
AU - Purwaningsih, Erni Hernawati
AU - Krisnamurti, Desak Gede Budi
N1 - Publisher Copyright:
© 2021 Author(s).
PY - 2021/3/23
Y1 - 2021/3/23
N2 - Aging is a time-related process leading to progressive deterioration of physiological bodily functions and increased vulnerability to degenerative disorders. The current trend of rapid growth in the global aging population poses a challenge for healthcare due to the increasing incidence of chronic diseases. In spite of this, preventive anti-aging agents such as vitamin supplements are not adequately available in many countries. Centella asiatica (CA), a traditional herb native to Southeast Asia, has been widely studied and demonstrated potent anti-inflammatory, antioxidant, neuroprotective, wound healing, and vasculoprotective effects in clinical studies. This study investigates the effect of CA treatment on aged Sprague-Dawley (SD) rats. Twenty-four rats were divided into four groups: positive control (vitamin E 6 IU), negative control (placebo), treatment group (CA 300 mg/kgBW), and comparison group (young SD rats with placebo). After 28 days of treatment, SD rats were terminated. TNF-a concentration in heart and kidney tissues were determined using enzyme-linked immunosorbent assay. We found that CA showed a significant decrease in heart TNF-alpha (p = 0.021) yet yields no statistically significant decrease in kidney TNF-alpha levels (p = 0.617). However, it was possible to identify a trend toward decreasing kidney TNF-a concentration in groups treated with CA as it was lower than the positive and negative control group. Our finding suggests different aging pathophysiology in different body organs and CA's potential as an anti-aging agent, corresponding to previous studies showing potent anti-inflammatory effects of CA in multiple organs. All in all, further research and exploration need to be made on aging pathophysiology and CA with variations of a more significant dose and longer time of administration.
AB - Aging is a time-related process leading to progressive deterioration of physiological bodily functions and increased vulnerability to degenerative disorders. The current trend of rapid growth in the global aging population poses a challenge for healthcare due to the increasing incidence of chronic diseases. In spite of this, preventive anti-aging agents such as vitamin supplements are not adequately available in many countries. Centella asiatica (CA), a traditional herb native to Southeast Asia, has been widely studied and demonstrated potent anti-inflammatory, antioxidant, neuroprotective, wound healing, and vasculoprotective effects in clinical studies. This study investigates the effect of CA treatment on aged Sprague-Dawley (SD) rats. Twenty-four rats were divided into four groups: positive control (vitamin E 6 IU), negative control (placebo), treatment group (CA 300 mg/kgBW), and comparison group (young SD rats with placebo). After 28 days of treatment, SD rats were terminated. TNF-a concentration in heart and kidney tissues were determined using enzyme-linked immunosorbent assay. We found that CA showed a significant decrease in heart TNF-alpha (p = 0.021) yet yields no statistically significant decrease in kidney TNF-alpha levels (p = 0.617). However, it was possible to identify a trend toward decreasing kidney TNF-a concentration in groups treated with CA as it was lower than the positive and negative control group. Our finding suggests different aging pathophysiology in different body organs and CA's potential as an anti-aging agent, corresponding to previous studies showing potent anti-inflammatory effects of CA in multiple organs. All in all, further research and exploration need to be made on aging pathophysiology and CA with variations of a more significant dose and longer time of administration.
KW - aging
KW - Centella asiatica
KW - oxidative stress
KW - tumor necrosis factor-alpha
UR - http://www.scopus.com/inward/record.url?scp=85103510292&partnerID=8YFLogxK
U2 - 10.1063/5.0048269
DO - 10.1063/5.0048269
M3 - Conference contribution
AN - SCOPUS:85103510292
T3 - AIP Conference Proceedings
BT - 5th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Lischer, Kenny
A2 - Supriadi, Sugeng
A2 - Rahman, Siti Fauziyah
A2 - Whulanza, Yudan
PB - American Institute of Physics Inc.
T2 - 5th International Symposium of Biomedical Engineering, ISBE 2020
Y2 - 28 July 2020 through 29 July 2020
ER -