Adipose-Derived Stem Cell Condition Medium Enhances Expression of Ephrin-B2 Related to Neovascularization in Burn Wound Tissue

The transmembrane ligand ephrin-B2 is molecular marker for arterial endothelial cells and early blood vessel formation. Neovascularization is one of the important event that support wound healing. Adiposederived stem cells conditioned medium (ADSC-CM) is known to contain cytokines and growth factors as regenerative agent. This study aims to investigate the effect of ADSC-CM on ephrin-B2 expression that represent as neovascularization marker in burn wound granulation tissue. Male Sprague dawley rats were randomly divided into control, conditioned medium (CM) and medium complete (MC) groups. Full-thickness burn wounds were created by placing preheated metal plat on dorsal thorax of the prepared rats. The burn wounds were treated once daily according to their group. Skin wound tissues were collected at day 7, 14, and 21 post wounded for histological analysis. The expression of ephrin-B2 was demonstrated by immunohistochemistry staining. Neovascularization evaluation was done by calculating the number of newly formed capillaries that expressed Ephrin-B2 in wound granulation tissues. Expression of Ephrin-B2 in burn wound tissue was prominently increased in CM compared to control and MC groups on day 7, 14 and 21 post wounded. The CM group also showed higher neovascular number in granulation tissue compared to control and MC group on day 7, 14 and 21 (p < 0.05). Our results showed prominent ephrin-B2 expression in burn wound tissue after treated with ADSC-CM. Topical application of ADSC-CM has shown to promote angiogenesis in full-thickness burn wound in part through the increased of ephrin-B2 expression. Therefore ADSC-CM could act as an alternative strategy to promote burn wound healing.