Adhesion activity assay of Lactobacillus plantarum AKK30 combined with oligosaccharides

This objective of study was to measure the activity of lactic acid bacteria (LAB) adhesions in the intestinal mucosa. Those of lactic acid bacteria used were L. plantarum AKK 30 and were combined either with inulin oligosaccharides (OS) or mannan oligosaccharides (MOS). The oligosaccharides concentration used in this experiment were, 0%, 0.5%, 1%, 1.5% and 2%, respectively. Data collected were total plate count (TPC) before and after adhesion assay. The microstructure of L. plantarum AKK 30 was observed by scanning electron microscope (SEM). The TPC results of the combination of L. plantarum and inulin (OS) were 18 × 10 ⁷ (0%), 48 × 10 ⁷ (0.5%),109 × 10 ⁷ (1.0%), 129 × 10 ⁷ (1.5%), and 99 × 10 ⁷ (2.0%), respectively, while the combination of L. plantarum and mannan were 18 × 10 ⁷ (0%), 53 × 10 ⁷ (0.5%),125 × 10 ⁷ (1.0%), 210 × 10 ⁷ (1.5%), and 66 × 10 ⁷ (2.0%), respectively. The highest growth average of LAB were shown by the combination L. plantarum and 1.5% of MOS. In addition, the TPC results of assay adhesion showed that the combination of L. plantarum and inulin oligosaccharides were: 3.89 × 10 ⁸ (0.5%), 4.39x 10 ⁸ (1.0%), 3.19 × 10 ⁸ (1.5%), and 5.09 × 10 ⁸ (2.0%), respectively, whereas the combination of L. plantarum and mannan were: 7.29 × 10 ⁸ (0.5%), 15.49 × 10 ⁸ (1.0%), 2.89 × 10 ⁸ (1.5%), and 4.49 × 10 ⁸ (2.0%), respectively. There were significant differences in the TPC results of assay adhesion among the treatment groups and control (1.16 × 10 ⁸ ). The highest activity of LAB adhesion was shown by the combination of L. plantarum and 1% of mannan. The TPC result was supported by the SEM observation. The combination of L. plantarum with oligosaccharides affects the growth and the adhesion length of lactic acid bacteria in the intestine. Further, the optimum concentration was shown by 1.0% of mannan combined with L. plantarum.